Nucleic Acids Research, 1992, Vol. 20, No. 1 111-116
© 1992
MOLECULAR BIOLOGY |
A CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif
Department of Human Genetics, Roswell Park Cancer Institute Elm and Carlton Streets, Buffalo, NY 14263, USA
*To whom correspondence should be addressed
Received September 10, 1991. Revised November 22, 1991. Accepted November 22, 1991.
Numerous genes contain promoter elements that are nuclease hypersensitive. These elements frequently possess polypurine/polypyrimidine stretches and are usually associated with altered chromatin structure. We have previously isolated a clone that binds a class of CT-rich promoter elements. We have further characterized this clone, termed the nuclease-sensitive element profein-1, or NSEP-1. NSEP-1 binds both duplex CT elements and the CT-rich strand of these elements fin a ‘generic’ sequence specific manner and has overlapping but distinct single-and double-strand DNA binding domains. The minimal peptide region sufficient for both duplex and single-strand DNA binding includes two regions rich in basic amino acids flanking an RNP-CS-1 like octapeptide motif. Deletion analysis shows that the single-strand DNA binding activity is mediated by the RNP-CS-1 like octapeptide motif and is the key peptide region necessary for single-strand binding. NSEP-1's affinity for CT rich promoter elements with strand asymmetry in addition to its double- and single-strand DNA binding properties suggests that it may be a member of a class of DNA binding proteins that modulate gene expression by their ability to recognize DNA with unusual secondary structure.
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