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Nucleic Acids Research, 1992, Vol. 20, No. 11 2853-2859
© 1992

MOLECULAR BIOLOGY

Identification of an enhancer involved in the melanoma-specific expression of the tumor antigen melanotransferrin gene

Nathalie Duchange, Alberto Ochoa, Gregory D. Plowman1, Anne Rozé, Mardjan Amdjadi and Mario M. Zakin

Laboratoire d'Expression des Gènes Eucaryotes, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France 1 Oncogen, 3005 First Avenue, Seattle, WA 98121, USA

Received January 17, 1992. Revised April 27, 1992. Accepted April 27, 1992.

Melanotransferrin (MTf) is a tumor associated antigen found in abundance on the surface of melanoma cells. It is a transferrin-like protein found in low amount in most adult tissues and whose gene is reminiscent of house-keeping genes. With the goal of understanding the regulatory mechanisms which might explain the enhancement of expression in tumor cells, we report here the characterization of a regulatory element located 2 kbp upstream from the promoter and whose deletion specifically impairs gene expression in melanoma cells; we show that this element is part of an enhancer composed of two modules which are each the target for the AP1 transcription factor. The two modules present a synergistic mode of action specific for melanoma cells which requires both of the 130 bp away AP1 sites. Furthermore, we show that the enhancer behaves differently according to the promoter context.


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