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Nucleic Acids Research, 1992, Vol. 20, No. 13 3333-3339
© 1992

MOLECULAR BIOLOGY

The AT-rich tract of the SV40 oh core: negative synergism and specific recognition by single stranded and duplex DNA binding proteins

Ivo Galli, Sanae M.M. Iguchi-Ariga1 and Hiroyoshi Ariga*

Faculty of Pharmaceutical Sciences Kita-ku, N12 W6, Sapporo 060, Japan 1College of Medical Technology, Hokkaido University Kita-ku, N12 W6, Sapporo 060, Japan

* To whom correspondence should be addressed

Received April 16, 1992. Revised June 15, 1992. Accepted June 15, 1992.

The SV40 origin of replication comprises a run of thymine and adenine residues. Integrity of this AT-rich sequence is known to be essential for replication. We set out to study whether or not these elements can work synergistically to sustain replication. Quite surprisingly, additional copies of the AT stretch linked to a functional SV40 ori core dramatically reduce its replication in Cosl cells, probably by creating some physical block. Interestingly, the same inhibiting effect can be observed with the addition in cis of the yeast ARS consensus, which is homologous to the SV40 AT stretch. This modulation is possibly due to the action of cellular factors that recognize either of the two sequences. In fact, we demonstrate the existence of factor(s) in Cosl crude nuclear extracts that in vitro can specifically bind to either of them. Moreover, we show that these sequence-specific factors) (MW about 50 kDa), named SOAP, recognize both single (T-rich strand) and double stranded forms of the AT tracts. Binding to single stranded AT stretches can be specifically inhibited by the corresponding duplex form, but not vice versa.


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