Nucleic Acids Research, 1992, Vol. 20, No. 13 3479-3483
© 1992
MOLECULAR BIOLOGY |
A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae
Microbiologie et Génétique Moléculaires, CNRS-UPR 9007, Université Paul Sabatier 118 route de Narbonne, 31062 Toulouse Cedex, France 1Department of Microbiology, University of Leicester PO Box 138, Medical Sciences Building, University Road, Leicester LE1 9HN, UK 2Max-Planck Institut für Molekulare Genetik Ihnestraße 73, D-1000 Berlin 33, Germany 3Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago Chicago, IL 60680, USA
* To whom correspondence should be addressed
Received February 10, 1992. Revised June 12, 1992. Accepted June 12, 1992.
We report the discovery of a group of highly conserved DNA sequences located, in those cases studied, within intergenic regions of the chromosome of the Gram positive Streptococcus pneumoniae. The S. pneumoniae genome contains about 25 of these elements called BOX. From 5' to 3', BOX elements are composed of three subunits (boxA, boxB, and boxC) which are 59, 45 and 50 nucleotides long, respectively. BOX elements containing one, two and four copies of boxB have been observed; boxB alone was also detected in one instance. These elements are unrelated to the two most thoroughly documented families of repetitive DNA sequences present in the genomes of enterobacteria. BOX sequences have the potential to form stable stem-loop structures and one of these, at least, is transcribed. Most of these elements are located in the immediate vicinity of genes whose product has been implicated at some stage in the process of genetic transformation or in virulence of S. pneumoniae. This location raises the intriguing possibility that BOX sequences are regulatory elements shared by several coordinately controlled genes, including competence-specific and virulence-related genes.
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