Skip Navigation

This Article
Right arrow Print PDF (1145K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wang, T.W.
Right arrow Articles by Small, G.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, T.W.
Right arrow Articles by Small, G.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 13 3495-3500
© 1992

MOLECULAR BIOLOGY

A negative regulating element controlling transcription of the gene encoding acyl-CoA oxidase in Saccharomyces cerevisiae

T.W. Wang1, A.S. Lewin1 and G.M. Small2,*

Department of Anatomy and Cell Biology Gainesville FL 32610 1Department of Immunology and Medical Microbiology, University of Florida Gainesville FL 32610 2Department of Cell Biology/Anatomy, Mount Sinai School of Medicine New York, NY 10029, USA

*To whom correspondence should be addressed

Received January 21, 1992. Revised May 7, 1992. Accepted May 7, 1992.

Peroxisomes are induced in Saccharomyces carevisiae when this yeast is grown in the presence of oleate, and are repressed when glucose is supplied as the carbon source. Concomitant with this is an induction/repression of peroxisomal ß-oxidation enzymes. We are investigating the transcriptional control of acyl-CoA oxidase, the first and rate-limiting enzyme in the peroxisomai ß-oxidation cycle. The promoter region of POX1 from S.cerevisiae has been analyzed in POX1/lacZ fusions. Expression of the POX1/lacZ fusion protein underwent glucose repression and oleate induction. By deletion, DNA band shift and DNase I footprinting analyses we have identified a region that is involved in transcriptional repression of POX1. Elimination of this DNA sequence results in constitutive expression of POX1 when S.cerevisiae is grown on a fermentable carbon source or glycerol.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
I. V. Karpichev, L. Cornivelli, and G. M. Small
Multiple Regulatory Roles of a Novel Saccharomyces cerevisiae Protein, Encoded by YOL002c, in Lipid and Phosphate Metabolism
J. Biol. Chem., May 24, 2002; 277(22): 19609 - 19617.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
G. Parpinello, E. Berardi, and R. Strabbioli
A Regulatory Mutant of Hansenula polymorpha Exhibiting Methanol Utilization Metabolism and Peroxisome Proliferation in Glucose
J. Bacteriol., June 1, 1998; 180(11): 2958 - 2967.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
Y. Luo, I. V. Karpichev, R. A. Kohanski, and G. M. Small
Purification, Identification, and Properties of a Saccharomyces cerevisiae Oleate-activated Upstream Activating Sequence-binding Protein That Is Involved in the Activation of POX1
J. Biol. Chem., May 17, 1996; 271(20): 12068 - 12075.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.