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Nucleic Acids Research, 1992, Vol. 20, No. 15 3859-3864
© 1992

MOLECULAR BIOLOGY

The vacuum UV CD Spectra of G.G.C. triplexes

Kenneth H. Johnson*, Ross H. Durland+ and Michael E. Hogan

Baylor College of Medicine, The Center for Biotechnology 4000 Research forest Drive, The Woodlands, TX 77381, USA

*To whom correpondence should be addressed

Received May 27, 1992. Accepted July 3, 1992.

Vacuum UV circular dichroism (CD) spectra were measured down to 175 nm for d(C)10, d(G)10, the d(G)10-d(C)10 duplex, and the d(G)10-d(G)10-d(C)10 triplex. A CD difference spectrum was calculated for d(G)10-d(C)10 giving the change in CD induced by forming the duplex from d(G)10 and d(C)10. The d(G)10-d(G)10-d(C)10 CD difference spectrum gave the CD Induced by triplex formation from binding of d(G)10 to the d(G)10-d(C)10 duplex. In the near-UV, the d(G)10-d(C)10 and d(G)10-d(G)10-d(C)10 difference spectra resembled the difference spectrum for poly[r(G)-r(C)] (Blopolymers 29, 325-333). This similarity may be an indication of similar purine base stacking. The d(G)10-d(G)10-d(C)10 vacuum UV difference spectrum had a negative band at 195 nm and a positive band at 180 nm, making it similar to difference spectra for homopolymer triplexes containing T.A.T and U.A.U triplets (Nucl. Acids Res.19, 2275-2280). The appearance of these bands in difference spectra should be good Indicators of triplex formation. The complementary oligonucleotides c-myc/d(CCCCACCCTCCC) and c-mycll d(GGGAGGGTGGGG) are part of the regulatory sequences of the human c-myc gene. G-G-C rich triplexes formed by binding c-mycll or c-mycll I d(GGGGTGGGTGGG) to the c–myclc–mycll duplex had CD difference spectra similar to that of d(G)10-d(G)10-d(C)10 in both the vacuum UV and near UV regions, indicating similar triplet structures.

+Present address: Triplex Pharmaceutical corporation, 9391 Gorgans Mill Road, the Woddlands, TX 77380, USA


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 Proc. Natl. Acad. Sci. USAHome page
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