Skip Navigation

This Article
Right arrow Print PDF (1945K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kao, H.-Y.
Right arrow Articles by Siliciano, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kao, H.-Y.
Right arrow Articles by Siliciano, P. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 15 4009-4013
© 1992

MOLECULAR BIOLOGY

The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene

Hunh-Ying Kao and Paul G. Siliciano*

Department of Biochemistry and Institute of Human Genetics, University of Minnesota Medical School 4-225 Millard Hall, 435 Delaware Street SE, Minneapolis, MN 55455, USA

*To whom Correspondence should be addressed

Received June 30, 1992. The product of the yeast SNP1 gene has high homology to two domains of the metazoan U1 snRNP protein 70K, which binds to stem/loop I of the U1 RNA. However, the absence of other domains conserved in metazoan 70K and the minimal effect of yeast U1 RNA stem/loop I deletion make the assignment of SNP1 as yeast 70K less clear. To address this question, we have expressed the SNPl gene as a fusion protein in E. coli and developed a gel shift assay for U1 RNA binding. We show here that the product of the yeast SNP1 gene binds directly and specifically to the first 47 nucleotides of yeast U1 RNA, which include the stem/loop 1 structure. We therefore conclude that the SNP1 gene product is the yeast 70K homolog. This is the first yeast protein to be identified as a homolog of a metazoan snRNP protein.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Golovkin and A. S.N. Reddy
Expression of U1 Small Nuclear Ribonucleoprotein 70K Antisense Transcript Using APETALA3 Promoter Suppresses the Development of Sepals and Petals
Plant Physiology, August 1, 2003; 132(4): 1884 - 1891.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. Zhang and M. Rosbash
Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
Genes & Dev., March 1, 1999; 13(5): 581 - 592.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
H.-R. Chen, S.-P. Jan, T. Y. Tsao, Y.-J. Sheu, J. Banroques, and S.-C. Cheng
Snt309p, a Component of the Prp19p-Associated Complex That Interacts with Prp19p and Associates with the Spliceosome Simultaneously with or Immediately after Dissociation of U4 in the Same Manner as Prp19p
Mol. Cell. Biol., April 1, 1998; 18(4): 2196 - 2204.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Gee, S. W. Krauss, E. Miller, K. Aoyagi, J. Arenas, and J. G. Conboy
Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43
PNAS, October 28, 1997; 94(22): 11803 - 11807.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Awasthi, R. Palmer, M. Castro, C. D. Mobarak, and S. W. Ruby
New Roles for the Snp1 and Exo84 Proteins in Yeast Pre-mRNA Splicing
J. Biol. Chem., August 10, 2001; 276(33): 31004 - 31015.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.