Nucleic Acids Research, 1992, Vol. 20, No. 17 4429-4436
© 1992
MOLECULAR BIOLOGY |
Interaction of the erythroid transcription factor cGATA-1 with a critical auto-regulatory element
Department of Biological Sciences, Langley Hall Room 518, University of Pittsburgh, Pittsburgh, PA 15260, USA
*To whom correspondence should be addressed
Received June 23, 1992. Revised August 6, 1992. Accepted August 6, 1992.
We have performed a mutational analysis of the promoter for the chicken erythroid-specific GATA-1 transcription factor, and have investigated in detail the interaction of the factor with an upstream autoregulatory element (ARE). We find that a single proximal GATA binding site of the ARE is required for promoter activity in primary erythroid cells; however, this minimal promoter is inappropriately active in fibroblasts. At least two molecules of GATA-1 can interact with the ARE, and sequences outside of the consensus site appear critical for the transcriptional activity of the bound protein. Finally, we provide evidence for complex protein/DNA interactions at the ARE, including the ability of GATA-1 to bend DNA.
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