Skip Navigation

This Article
Right arrow Print PDF (3142K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Anin, M.-F.
Right arrow Articles by Marc, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anin, M.-F.
Right arrow Articles by Marc, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 18 4825-4830
© 1992

MOLECULAR BIOLOGY

Lability of monofunctional cis-platinum adducts: role of DNA double helix

Marie-France Anin, Frédéric Gaucheron and Leng Marc*

Centre de Biophysique Moléculaire CNRS, 1A, Avenue de la Recherche Scientiflque, 45071 Orléans cedex 2, France

*To whom correspondence should be addressed

Received June 15, 1992. Revised August 28, 1992. Accepted August 28, 1992.

Recently, we have shown that the adduct formed in the reaction between the platinum-triamine complex cis-[Pt(NH3)2(N7-N-methyl-2diazapyrenium)CI]2+ and one single-stranded oligonucleotide was stable but became labile as soon as the platinated oligonucleotide was paired with its complementary strand (Gaucheron et al. Proc. Natl. Acad. Sci. USA 88,3516–3519(1991)). To generalize this finding we have now studied large DNA fragments containing several adducts. The stability of the adducts within single-stranded DNA is demonstrated by absorption spectrophotometry and by replication mapping experiments. Several approaches are used to prove the lability of the adducts within double-stranded DNA. Replication mapping experiments reveal that an unmodified single-stranded DNA when mixed with double-stranded DNA modified by the platinum-triamine complex behaves as a singlestranded DNA modified by the triamine complex. After double-stranded DNA is modified by the platinum triamine complex, intrastrand and interstrand crosslinks are progressively formed during subsequent incubation as revealed by transcription mapping experiments and gel electrophoresis under denaturing conditions. Finally, replication mapping experiments show that the lability of the adducts within a doublestranded DNA depends upon the nature of the flanking nucleotide residues. All these results support the proposal that the DNA double helix acts as a catalyst in the reaction between DNA, cis-diamminedichioroplatinum(II) and N-methyl-2,7-diazapyrenium.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
M. Leng, D. Locker, M.-J. Giraud-Panis, A. Schwartz, F. P. Intini, G. Natile, C. Pisano, A. Boccarelli, D. Giordano, and M. Coluccia
Replacement of an NH3 by an Iminoether in Transplatin Makes an Antitumor Drug from an Inactive Compound
Mol. Pharmacol., April 13, 2001; 58(6): 1525 - 1535.
[Abstract] [Full Text]

Home page
 Mol. Pharmacol.Home page
O. Nováková, M. F. Sivo, G. Natile, and V. Brabec
DNA Interactions of a Novel Platinum Drug, cis-[PtCl(NH3)2(N7-Acyclovir)]+
Mol. Pharmacol., May 1, 1998; 53(5): 846 - 855.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.