Skip Navigation

This Article
Right arrow Print PDF (5597K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Date, T.
Right arrow Articles by Matsukage, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Date, T.
Right arrow Articles by Matsukage, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 18 4859-4864
© 1992

MOLECULAR BIOLOGY

Two regions in human DNA polymerase ß mRNA suppress translation in Escherichia coli

Takayasu Date, Kiyomi Tanihara, Setsuko Yamamoto, Nobuo Nomura1 and Akio Matsukage2

Department of Biochemistry and Medical Research Institute, Kanazawa Medical University, Uchinada Ishikawa 920–02 1Laboratory of Molecular Biology, Institute of Gerontology, Nippon Medical School 1–396 Nakahara, Kawasaki 211 2Laboratory of Cell Biology, Aichi Cancer Research Institute, Chikusa Nagoya 464, Japan

Received June 8, 1992. Revised August 28, 1992. Accepted August 28, 1992.

Although human DNA polymerase ß (DNA polß) shows 96% Identity with rat DNA pol at the amino acid level, it is weakly expressed in Escherlchla (E.) coli relative to the rat enzyme. The mechanism of this suppression was investigated. Pulse-chase protein labeling and steady state mRNA analysis showed that mature human DNA polß protein is relatively stable in E.coli and the levels of human and rat DNA polß mRNA were comparable indicating that the human DNA pol ß expression is suppressed at the translational level. By systematic expression analysis of a number of chimeric genes composed of human and rat cDNAs, two strong translational suppression regions were mapped in the human DNA polß mRNA; one was named TSR-1, corresponding to CGG encoding arginine (arg) at postition 4 and the other, termed TSR-2, is located between codons 153 and 199. SInce substitution of the rat Arg-4 codon with synonymous codons showed strong effects upon the expression level, we propose that the arg codon at the N-terminal cording region plays a role in modulating expression.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.