Skip Navigation

This Article
Right arrow Print PDF (7371K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (64)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Jayasena, S. D.
Right arrow Articles by Johnston, B. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jayasena, S. D.
Right arrow Articles by Johnston, B. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 20 5279-5288
© 1992

MOLECULAR BIOLOGY

OligonucleotJde-directed triple helix formation at adjacent oligopurine and oligopyrimidine DNA tracts by alternate strand recognition

Sumedha D. Jayasena+ and Brian H. Johnston*

Cell and Molecular Biology Laboratory, SRI International 333 Ravenswood Avenue, Menlo Park, CA 94025, USA

* To whom correspondence should be addressed

Received August 12, 1992. Revised September 22, 1992. Accepted September 22, 1992.

A significant limitation to the practical application of triplex DNA is its requirement for oligopurine tracts in target DNA sequences. The repertoire of triplex-forming sequences can potentially be expanded to adjacent blocks of purines and pyrimidines by allowing the third strand to pair with purines on alternate strands, while maintaining the required strand polarities by combining the two major classes of base triplets, Py · PuPy and Pu · PuPy. The formation of triplex DNA in this fashion requires no unusual bases or backbone linkages on the third strand. This approach has previously been demonstrated for target sequences of the type 5'-(Pu)n(Py)n-3' in intramolecular complexes. Using affinity cleaving and DNase I footprinting, we show here that intermolecular triplexes can also be formed at both 5'-(Pu)n(Py)n-3' and 5'-{Py)n(Pu)n-3' target sequences. However, triplex formation at a 5'-(Py)n(Pu)n-3' sequence occurs with lower yield. Triplex formation is disfavored, even at acid pH, when a number of contiguous C+ ·GC base triplets are required. These results suggest that triplex formation via alternate strand recognition at sequences made up of blocks of purines and pyrimidines may be generally feasible.

+ Present address: Nexagen, Inc., 2860 Wilderness Place, Boulder, CO 80301, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
J. Koch
Neocentromeres and alpha satellite: a proposed structural code for functional human centromere DNA
Hum. Mol. Genet., January 22, 2000; 9(2): 149 - 154.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Bouziane, D. I. Cherny, J.-F.ço. Mouscadet, and C. Auclair
Alternate Strand DNA Triple Helix-mediated Inhibition of HIV-1 U5 Long Terminal Repeat Integration in Vitro
J. Biol. Chem., April 26, 1996; 271(17): 10359 - 10364.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.