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Nucleic Acids Research, 1992, Vol. 20, No. 20 5345-5350
© 1992

MOLECULAR BIOLOGY

A spliced intron accumulates as a lariat in the nucleus of T cells

Lian Qian, Minh N. Vu, Mark Carter and Miles F. Wilkinson*

Vollum Institute for Advanced Biomedical Research, Microbiology & Immunology Department, Oregon Health Sciences University L220, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201, USA

* To whom correspondence should be addressed

Received July 20, 1992. Revised September 18, 1992. Accepted September 18, 1992.

The vast majority of mammalian genes are Interrupted by non-coding segments of DNA termed Introns. Introns are spliced out of RNA transcripts as lariat structures, and then are typically debranched and rapidly degraded. Here, we describe an unusual spliced intron from the constant region of the T cell receptor-ß (TCR-ß) locus that is relatively stable in mammalian cells. This intron, IVS1cß1, accumulates as a set of lariat RNA structures with different length talls in the nucleus of T cells. The accumulation of this spliced intron is developmentally regulated during murine thymocyte ontogeny. The property of stability appears to be evolutionary conserved since the human version of this intron also accumulates in T cells. The stability is selective since other spliced TCR-ß introns do not detectably accumulate in T cells. The unusual stability of this intron does not depend on T cell specific factors since non-T cells transfected with TCR-ß gene constructs also accumulate spliced IVSIcß1. The discovery of a mammalian intron that accumulates as a lariat in vivo provides an opportunity to elucidate mechanisms that regulate intron debranching, stability, and nuclear localization.


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