Skip Navigation

This Article
Right arrow Print PDF (2140K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Reddy, D. M.
Right arrow Articles by McCloskey, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reddy, D. M.
Right arrow Articles by McCloskey, J. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 21 5607-5615
© 1992

CHEMISTRY

Structure determination of two new amino acid-containing derivatives of adenosine from tRNA of thermophilic bacteria and archaea

Dasari M. Reddy, Pamela F. Crain, Charles G. Edmonds+, Ramesh Gupta1, Takeshi Hashizume, Karl O. Stetter2, Friedrich Widde3,§ and James A. McCloskey*

Departments of Medicinal Chemistry and Biochemistry, University of Utah Salt Lake City, UT 84112 1Southern Illinois University School of Medicine Carbondale, IL 62901 2Lehrstuhl für Mikrobiologie, Universität Regensburg Germany 3Department of Microbiology, University of Illinois Urbana, IL 61801, USA

* To whom correspondence should be addressed

Received August 25, 1992. Revised October 5, 1992. Accepted October 5, 1992.

Two new nucleosides have been identified in unfractionated transfer RNA of two thermophilic bacteria, Thermodesulfobacterium commune, and Thermotoga maritima, six hyperthermophlic archaea, including Pyrobaculum islandicum, Pyrococcus furiosus and Thermococcus sp. and two mesophilic archaea, Methanococcus vannielii and Methanolobus tindarius Structures were determined primarily by mass spectro-metry, as 3-hydroxy-/N-[[(9-ß-D-ribofuranosyl-9H- purin-6-yl)amino]carbonyl]norvaline, (hn6A), structure 1, and 3-hydroxy-N-[[(9-ß-D-ribofuranosyl-9H-2-methyl-thiopurin-6-yl)amino]carbonyl]norvaline (ms2hn6A), 2. The amino acid side chain was characterized as 3-hydroxynorvaline (3) by gas chromatography-mass spectrometry of the trimethylsilyl derivative after cleavage from 1 and 2 by alkaline hydrolysis. Evidence for the amino acid-purine carbamoyl linkage was obtained from the collision-induced dissociation mass spectrum of trimethylsilylated 1, and the total structure was confirmed bv chemical svnthesis of 1.

+Present addresses: Battelle Pacific Northwest Laboratories, Richland, WA 99352, USA

§Max-Planck-Institut für Marine Mikrobiologie, BITZ, Fahrenheitstrasse 1, W-2800 Bremen 33, Germany


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
B. P. Anton, L. Saleh, J. S. Benner, E. A. Raleigh, S. Kasif, and R. J. Roberts
RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli
PNAS, February 12, 2008; 105(6): 1826 - 1831.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. R. Noon, R. Guymon, P. F. Crain, J. A. McCloskey, M. Thomm, J. Lim, and R. Cavicchioli
Influence of Temperature on tRNA Modification in Archaea: Methanococcoides burtonii (Optimum Growth Temperature [Topt], 23{degrees}C) and Stetteria hydrogenophila (Topt, 95{degrees}C)
J. Bacteriol., September 15, 2003; 185(18): 5483 - 5490.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. A. McCloskey, D. E. Graham, S. Zhou, P. F. Crain, M. Ibba, J. Konisky, D. Soll, and G. J. Olsen
Post-transcriptional modification in archaeal tRNAs: identities and phylogenetic relations of nucleotides from mesophilic and hyperthermophilic Methanococcales
Nucleic Acids Res., November 15, 2001; 29(22): 4699 - 4706.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.