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Nucleic Acids Research, 1992, Vol. 20, No. 21 5779-5784
© 1992

ENZYMOLOGY

Delta-type DNA polymerase characterized from Drosophila melanogaster embryos

Vickie M. Peck1, Eugene W. Gerner1,2 and Anne E. Cress1,*

1Departments of Radiation Oncology, The Arizona Cancer Center, University of Arizona Tucson, AZ 85724, USA 2Departments of Biochemistry, The Arizona Cancer Center, University of Arizona Tucson, AZ 85724, USA

* To whom correspondence should be addressed

Received June 29, 1992. Revised September 28, 1992. Accepted September 28, 1992.

Genetic and biochemical evidence suggests there are at least three DNA polymerases required for replication in eukaryotic cells. However, Drosophila embryonic cells have a very short duration S phase which is regulated differently. To address the question of whether embryos utilize different DNA polymerases, we employed Mono Q anion exchange chromatography to resolve the DNA polymerase activities. Two types of DNA polymerase, DNA polymerase {delta} and DNA polymerase {alpha}, were distinguished by: 1. copurification of DNA primase or 3'–5'exonuclease activities; 2. immunoblot analysis with {alpha}-specific polyclonal antisera; 3. sensitivity to aphidicolin and BuPdGTP; and 4. processivity measurements with and without Proliferating Cell Nuclear Antigen. These observations suggest that Drosophila embryos, similar to nonembryonic cells, have both {alpha}- and {delta}-type DNA polymerases.


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