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Nucleic Acids Research, 1992, Vol. 20, No. 22 5927-5935
© 1992

MOLECULAR BIOLOGY

The FLP protein contacts both major and minor grooves of its recognition target sequence

Gagan B. Panigrahi, Linda G. Beatty and Paul D. Sadowski*

Department of Molecular and Medical Genetics, Medical Sciences Building, University of Toronto Toronto, Ontario M5S 1A8, Canada

* To whom correspondence should be addressed

Received September 4, 1992. Revised October 10, 1992. Accepted October 10, 1992.

The FLP protein of the 2µm plasmid of Saccharomyces cerevisiae promotes conservative site-specific recombination between DNA sequences that contain the FLP recognition target (FRT). FLP binds to each of the three 13 base pair symmetry elements in the FRT site in a site-specific manner. We have probed both major and minor groove contacts of FLP using dimethyl sulphate, monoacetyl-4-hydroxyaminoquinoline 1-oxide and potassium permanganate and find that the protein displays extensive interactions with residues of both the major and minor grooves of 10 base pairs of each symmetry element. We find no evidence that the FRT site assumes a single-stranded conformation upon FLP binding.


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X.-D. Zhu and P. D. Sadowski
The Role of Single-stranded DNA in Flp-mediated Strand Exchange
J. Biol. Chem., February 27, 1998; 273(9): 4921 - 4927.
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