Skip Navigation

This Article
Right arrow Print PDF (8470K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lauderdale, J. D.
Right arrow Articles by Stein, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lauderdale, J. D.
Right arrow Articles by Stein, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 24 6589-6596
© 1992

MOLECULAR BIOLOGY

Introns of the chicken ovalbumin gene promote nucleosome alignment in vitro

James D. Lauderdale+ and Arnold Stein*

Department of Biological Sciences, Purdue University West Lafayette, IN 47907, USA

*To whom correspondence should be addressed

Received September 11, 1992. Revised October 25, 1992. Accepted October 25, 1992.

A defined in vitro chromatin assembly system was used to examine the nucleosome alignment induced by histone H5 throughout a 12 kilobase pair chicken genomic DNA fragment containing the ovalbumin gene. In contrast with total fragmented chicken DNA and several anonymous cloned fragments, much of the gene permitted histone H5 to space nucleosomes at physiological intervals in an extended array. Nucleosomes at the 3'-end of the gene and on approximately 4 kilobase pairs of 5'-flanking ovalbumin sequence did not become aligned to appreciable extents. Analysis of cloned 2–3 kilobase pair subfragments suggested that a strong nucleosome alignment signal, specifying a 196 ± 5 base pair repeat exists in intron E. A second discrete region of the gene, which mapped approximately to intron A, exhibited nucleosome alignment with a spacing periodicity of about 200 base pairs. The ovalbumin cDNA did not permit nucleosome alignment. These findings suggest that some of the introns contain signals that direct nucleosome alignment over the ovalbumin gene in a way conducive to its regulation.

+Present address: Department of Biology, University of Michigan, Ann Arbor, MI 48109, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
X. Hong, D. G. Scofield, and M. Lynch
Intron Size, Abundance, and Distribution within Untranslated Regions of Genes
Mol. Biol. Evol., December 1, 2006; 23(12): 2392 - 2404.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
A. E. Vinogradov
"Genome design" model: Evidence from conserved intronic sequence in human-mouse comparison
Genome Res., March 1, 2006; 16(3): 347 - 354.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. E. Vinogradov
Noncoding DNA, isochores and gene expression: nucleosome formation potential
Nucleic Acids Res., January 26, 2005; 33(2): 559 - 563.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. E. Vinogradov
DNA helix: the importance of being GC-rich
Nucleic Acids Res., April 1, 2003; 31(7): 1838 - 1844.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Stein, Y. Dalal, and T. J. Fleury
Circle ligation of in vitro assembled chromatin indicates a highly flexible structure
Nucleic Acids Res., December 1, 2002; 30(23): 5103 - 5109.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
A. E. Vinogradov
Bendable Genes of Warm-blooded Vertebrates
Mol. Biol. Evol., December 1, 2001; 18(12): 2195 - 2200.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Karrer and T. A. VanNuland
Nucleosome Positioning Is Independent of Histone H1 in Vivo
J. Biol. Chem., November 12, 1999; 274(46): 33020 - 33024.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.