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Nucleic Acids Research, 1992, Vol. 20, No. 3 521-523
© 1992

MOLECULAR BIOLOGY

Srand specific PCR amplification of low copy number DNA

Andreas Meyerhans{dagger}, Jean-Pierre Vartanian and Simon Wain-Hobson*

Laboratoire de Rétrovirologie Moléculaire, Institut Pasteur 28 Rue de Dr. Roux, 75724 Paris Cedex 15, France

{dagger} Present address: Department of Virology, Institut for Medical Microbiology and Hygiene, University of Freiburg, D-7800 Freiburg, FRG

Received October 3, 1991. Revised February 7, 1992. Accepted February 7, 1992.

A method for the amplification of a single DMA strand at Dow copy number is described. lit is a wholly PCR based approach which involves aim initial linear amplification of the target using a tagged strand specific primer. This is followed by classical PCR amplification of the progeny using a pair of primers, one specific for the sequence tagged onto the 5' end of the first round primer, the second specific for the target sequence. Given the protocol used the ratio of the two strands in the final amplification product was 50:1.


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