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Nucleic Acids Research, 1992, Vol. 20, No. 4 645-651
© 1992

ENZYMOLOGY

Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans

Sean M. Baker+, Geoffery P. Margison1 and Peter Strike*

Department of Genetics and Microbiology, University of Liverpool PO Box 147, Liverpool, L69 3BX 1CRC Department of Chemical Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute Manchester M20 9BX, UK

*To whom correspondence should be addressed

Received January 10, 1992. Accepted January 24, 1992.

We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nKrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells Increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5kDa and 21kDa, both of which have activity primarily against O6-methylguanine (06-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21kDa proteins. The other, a protein of 19.5kDa, Is highly inducible and can only be detected in treated cells. Unlike the other three proteins, It acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi.

+Present address: Department of Therapeutic Radiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA


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