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Nucleic Acids Research, 1992, Vol. 20, No. 4 797-803
© 1992

Articles

Tissue restricted expression and chromosomal localization of the YB-1 gene encoding a 42kD nuclear CCAAT binding protein

Dimitri D. Spitkovsky+, Brigitte Royer-Pokora1, Hajo Delius, Fjodor Kisseljov2, Nancy A. Jenkins3, Debra J. Gilbert3, Neal G. Copeland3 and Hans-Dieter Royer*

Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506 D 6900 Heidelberg, Russia 1Institut fur Humangenetik und Anthropologie der Universitaät Heidelberg FRG, Russia 2All Union Cancer Research Center 115478 Moscow, Russia 3Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center Frederick, MD 21702, USA

*To whom correspondence should be addressed

Received November 11, 1991. Revised January 24, 1992. Accepted January 24, 1992.

YB-1 cDNA clones were isolated by binding site screening of a Hela expression library using a human papillomavirus type 18 enhancer oligonucleotlde. YB-1 belongs to a family of transcription factors which bind to recognition sequences containing a core CCAAT element. YB-1 bound to its single stranded recognition sequence on the sense strand but not to the anti-sense strand. A synthetic peptide antiserum derived from the predicted YB-1 amino acid sequence identified a 42kD nuclear protein in immunoblots. A protein with the same size was detected by binding site blotting experiments using the HPV18 enhancer oligonucleotide which bound YB-1. YB-1 gene expression was restricted in tissues from a human 24 week old fetus. High levels of YB-1 mRNA were present in heart, muscle, liver, lung, adrenal gland and the brain, in contrast, low amounts of YB-1 mRNA were found in thymus, kidney, bone marrow and spleen. In pancreas, bladder, stomach and testis YB-1 mRNA could not be detected by Northern hybridization. Finally, we have identified four YB-1 related loci in the mouse genome and have mapped these loci to four different mouse chromosomes by interspecific backcross analysis.

+On leave from the All Union Cancer Research Center, Moscow, Russia


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