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Nucleic Acids Research, 1992, Vol. 20, No. 7 1573-1578
© 1992

MOLECULAR BIOLOGY

The proximal promoter and the start site cooperate to specify correct U1 snRNA transcription initiation by RNA polymerase II

Alain Lescure, Sylvie Murgo, Philippe Carbon and Alain Krol

Unité ‘Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance’, Institut de Biologie Moléculaire et Cellulaire du CNRS 15, Rue Descartes, 67084 Strasbourg Cedex, France

Received January 16, 1992. Revised March 2, 1992. Accepted March 2, 1992.

In this work, we attempted to gain insight into the detailed mechanism allowing correct transcription initiation of U1 snRNA genes by RNA polymerase II. Abolition of the CA motif residing at –1/+1 in the Xenopus U1 gene leads to a loss of the ability of the promoter to direct accurate initiation. A discrete site is selected only if a purine preceded by a pyrimidine is positioned at 58/57 bp downstream of the center of the PSE. The PSE alone is unable to designate a discrete initiation site. Rather, It serves to set the location of an initiation window without discriminating suitable from unsuitable initiation sites. The latter role is devoted to a PyPu sequence positioned at –1/+1. Therefore, it is the concomitant action of the PSE and an essential PyPu positioned at the proper distance from this promoter that specifies correct U1 snRNA transcription initiation by RNA polymerase II.


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