Nucleic Acids Research, 1992, Vol. 20, No. 9 2355-2359
© 1992
MOLECULAR BIOLOGY |
v-Fps-responsiveness in the Egr-1 promoter is mediated by serum response elements
The Institute for Biomolecular Structure and Function and The Department of Biological Sciences, The Hunter College of The City University of New York 695 Park Avenue, New York, NY 10021 1Department of Medicine, Molecular Genetics and Cell Biology and Howard Hughes Medical Institute, University of Chicago Chicago, IL 60637, USA
*To whom correspondence should be addressed
Received December 12, 1991. Revised March 27, 1992. Accepted March 27, 1992.
Egr-1, a mitogen-responsive transcription factor, is rapidly induced by v-Fps in the absence of protein synthesis. Thus, Egr-1 is a primary response to the protein-tyrosine kinase activity of v-Fps. To determine the v-Fps-responsive elements in the Egr-1 promoter, deletion mutants of the Egr-1 promoter were used in transient expression assays. A v-Fps expression vector was cotransfected into NIH 3T3 cells with chloramphenicol acetyl transferase (CAT) gene expression vectors under the control of the Egr-1 promoter or the Egr-1 promoter containing various deletions. Responsiveness to v-Fps was restricted to a region that contained repeated CC(A/T)6GG sequences, known as CArG boxes. CArG boxes form the core of serum response element (SREs). v-Fps induced Egr-1 promoter activation was lost by sequential removal of four tandemly repeated SREs. This region, containing four SREs, was found to be sufficient for maximal Egr-1 induction by v-Fps when placed upstream from a heterologous promoter. Individual SREs from this region were able to respond to v-Fps, however, the activation of the individual SREs was lower than that observed for the clustered SREs. These data suggest that v-Fps-responsiveness in the Egr-1 promoter is mediated by SREs.
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