Nucleic Acids Research, 1992, Vol. 20, No. 9 2361-2366
© 1992
MOLECULAR BIOLOGY |
Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRP
Department of Medicine, Baylor College of Medicine and Veterans Affairs Medical Center Houston, TX 77030 1Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine Houston, TX 77030 2Department of Medical Specialties, M.D. Anderson Cancer Center Houston, TX 77030, USA
Received November 20, 1991. Revised March 23, 1992. Accepted March 23, 1992.
Transcripts derived from the 6 exon CALC I gene are differentially processed in a tissue-specific fashion to include or exclude a calcitonin-specific exon 4. All cell types which transcribe a second calcitonin/CGRP gene, CALC II, exclude exon 4. Substitution of the first 30 nucleotides of CALC I exon 4 with analogous CALC II sequence was sufficient to prevent recognition of exon 4 in in vitro or in vivo RNA splicing systems. UV crosslinking detected a 
66 kDa RNA-binding protein in HeLa nuclear extract which interacted with CALC I proximal exon sequence, but not CALC II or mutant sequences. UV crosslinking of this protein was inhibited by addition of nuclear extract from a cell type which normally causes exclusion of exon 4. These results identify an important regulatory element within exon 4 and support a model in which calcitonin production requires protein interaction with this sequence to facilitate exon recognition.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Shen, Y. Liu, L. Liu, C. Liang, and Q. Q. Li Unique Features of Nuclear mRNA Poly(A) Signals and Alternative Polyadenylation in Chlamydomonas reinhardtii Genetics, May 1, 2008; 179(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Messina, D. M. T. Yu, G. W. Both, P. L. Molloy, and B. G. Robinson Calcitonin-Specific Transcription and Splicing Targets Gene-Directed Enzyme Prodrug Therapy to Medullary Thyroid Carcinoma Cells J. Clin. Endocrinol. Metab., March 1, 2003; 88(3): 1310 - 1318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lou and R. F. Gagel Alternative Ribonucleic Acid Processing in Endocrine Systems Endocr. Rev., April 1, 2001; 22(2): 205 - 225. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Zhao, L. Hyman, and C. Moore Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 405 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lou, D. M. Helfman, R. F. Gagel, and S. M. Berget Polypyrimidine Tract-Binding Protein Positively Regulates Inclusion of an Alternative 3'-Terminal Exon Mol. Cell. Biol., January 1, 1999; 19(1): 78 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lou, K. M. Neugebauer, R. F. Gagel, and S. M. Berget Regulation of Alternative Polyadenylation by U1 snRNPs and SRp20 Mol. Cell. Biol., September 1, 1998; 18(9): 4977 - 4985. [Abstract] [Full Text]
|
|
|
|
|
|
R. P. Carstens, W. L. McKeehan, and M. A. Garcia-Blanco An Intronic Sequence Element Mediates Both Activation and Repression of Rat Fibroblast Growth Factor Receptor 2 Pre-mRNA Splicing Mol. Cell. Biol., April 1, 1998; 18(4): 2205 - 2217. [Abstract] [Full Text]
|
|
|
|
 |
|
 H Lou, R F Gagel, and S M Berget An intron enhancer recognized by splicing factors activates polyadenylation. Genes & Dev., January 15, 1996; 10(2): 208 - 219. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Norton Alternative pre-mRNA splicing: factors involved in splice site selection J. Cell Sci., January 1, 1994; 107(1): 1 - 7. [PDF]
|
|
|
|
|
|
A Lavigueur, H La Branche, A R Kornblihtt, and B Chabot A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding. Genes & Dev., December 1, 1993; 7(12a): 2405 - 2417. [Abstract] [PDF]
|
|
|
|
|
|
Q Sun, A Mayeda, R K Hampson, A R Krainer, and F M Rottman General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes & Dev., December 1, 1993; 7(12b): 2598 - 2608. [Abstract] [PDF]
|
|
|
|
|
|
A Watakabe, K Tanaka, and Y Shimura The role of exon sequences in splice site selection. Genes & Dev., March 1, 1993; 7(3): 407 - 418. [Abstract] [PDF]
|
|