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Nucleic Acids Research, 1993, Vol. 21, No. 24 5595-5599
© 1993

MOLECULAR BIOLOGY

Structure specific ds&2.urule;ss-RNase activity in the extreme halophile Halobacterium salinarium

Pelle Stolt and Wolfram Zillig

Maz-Planck-lnstitut fur Biochemie D-82152 Martinsried, Germany

Received September 21, 1993. Revised November 1, 1993. Accepted November 1, 1993.

A ds&2.urule;ss-RNA processing activity involved in antisense-RNA mediated gene regulation in the extremely halophilic archaebacterium Halobacterium salinarium was investigated in vivo. H.salinarium cells were transformed with DNA encoding an RNA species complementary to a part of the major lytic transcript, termed T4, of the H.salinarium phage øH. The transformants transcribing this construct, when infected by phage were able to process T4 in a similar way to the processing of the lytic transcript denoted T1, in the natural sense-antisense system. Processing of T4 was not observed under normal phage growth on wild-type cells. Thus the antisense-RNA mediated processing activity earlier reported is dependent on the presence of an RNA duplex and is not sequence specific.


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