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Nucleic Acids Research, 1994, Vol. 22, No. 14 2784-2790
© 1994

MOLECULAR BIOLOGY

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase

Magali Frugier, Catherine Florentz, Mir Wais Hosseini1, Jean-Marie Lehn2 and Richard Giegé*

UPR 'Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance' Institut de Biologie Moléculaire et Cellulaire du CNRS 15 rue René Descartes, F-67084 Strasbourg Cedex 1Laboratoire de Chimie de Coordination Organique, Institut Le Bel 4 rue Blaise Pascal, F-67000 Strasbourg Cedex, France 2Laboratoire de Chimie Supramoléculaire Institut Le Bel, 4 rue Blaise Pascal, F-67000 Strasbourg Cedex, France

*To whom correspondence should be addressed

Received April 13, 1994. Revised June 22, 1994. Accepted June 22, 1994.

The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts 25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38JN6C10.


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