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Nucleic Acids Research, 1994, Vol. 22, No. 15 2945-2950
© 1994

MOLECULAR BIOLOGY

The two duplicated insecticyanin genes, ins-a and ins-b are differentially expressed in the tobacco hornworm, Manduca sexta

Wan-Cheng Li* and Lynn M. Riddiford

Department of Zoology, University of Washington Seattle, WA 98195, USA

*To whom correspondence should be addressed at: Laboratory of Biochemistry and Molecular Biology, Harkness Eye Institute, Research Division, 5th Floor, Room 503, College of Physicians and Surgeons of Columbia University, 160 Fort Washington Avenue, New York, NY 10032, USA

Received May 23, 1994. Accepted June 24, 1994.

Two gene-specific probes were generated from the unique sequences in the 3' non-coding regions of the two insecticyanin genes, ins-a and ins-b to study the developmental expression of these genes in Manduca sexta. Both genes were initially transcribed in the freshly hatched first instar larvae and then expressed in the epidermis and to a lesser degree in the fat body during every larval feeding stage. In the epidermis of the 4th and 5th instar larvae, both mRNAs appeared shortly before ecdysis and accumulated to maximal levels within a day. As the larval epidermis became pupally committed on day 3 of the 5th (final) instar, INSa mRNA quickly decreased, whereas INS-b mRNA showed a second peak of accumulation. In the fat body, both genes showed a similar expression pattern within the 4th instar to that of the epidermis except that levels were lower and ins-b mRNA dominated. In the final instar, only ins-b mRNA was present in significant amount. These findings not only reveal that the two duplicated insecticyanin genes have diverged in their expression pattern but also demonstrate, for the first time, that fat body also expresses insecticyanin genes.


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