Nucleic Acids Research, 1994, Vol. 22, No. 15 3011-3017
© 1994
MOLECULAR BIOLOGY |
The yeast Saccharomyces cerevisiae DNA polymerase IV: possible involvement in double strand break DNA repair
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University 3-1 Yamada-Oka, Suita, Osaka 565, Japan 1laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health PO Box 12233, Research Triangle Park, NC 27709, USA
*To whom correspondence should be addressed
Received May 13, 1994. Revised July 13, 1994. Accepted July 13, 1994.
We identified and purified a new DNA polymerase (DNA polymerase IV), which is similar to mammalian DNA polymerase (3, from Saccharomyces cerevisiae and suggested that it is encoded by YCR14C (POLX) on chromosome III. Here, we provided a direct evidence that the purified DNA polymerase IV is indeed encoded by POLX. Strains harboring a pol4 deletion mutation exhibit neither mitotic growth defect nor a meiosis defect, suggesting that DNA polymerase IV participates in nonessential functions in DNA metabolism. The deletion strains did not exhibit UV- sensitivity. However, they did show weak sensitivity to MMStreatment and exhibited a hyper-recombination phenotype when intragenic recombination was measured during meiosis. Furthermore, MATa pol4A segregants had a higher frequency of illegitimate mating with a MAT
tester strain than that of wild-type cells. These results suggest that DNA polymerase IV participates in a double-strand break repair pathway. A 3.2kb of the POL4 transcript was weakly expressed in mitotically growing cells. During meiosis, a 2.2 kb POL4 transcript was greatly induced, while the 3.2 kb transcript stayed at constant levels. This induction was delayed in a swi4
strain during meiosis, while no effect was observed in a swi6 strain.
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