Nucleic Acids Research, 1994, Vol. 22, No. 16 3312-3316
© 1994
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Human xeroderma pigmentosum group G gene encodes a DNA endonuclease
Sealy Center for Molecular Science UTMB, 6.104 Medical Research Building, Galveston, TX 77555-1061, USA
*To whom correspondence should be addressed
Received June 19, 1994. Revised June 7, 1994. Accepted July 7, 1994.
Because of defective nucleotide excision repair of ultraviolet damaged DNA, xeroderma pigmentosum (XP) patients suffer from a high incidence of skin cancers. Cell fusion studies have identified seven XP complementation groups, A to G. Previous studies have implicated the products of these seven XP genes in the recognition of ultraviolet-induced DNA damage and in incision of the damage-containing DNA strand. Here, we express the XPG-encoded protein in Sf9 insect cells and purify it to homogeneity. We demonstrate that XPG is a single-strand specific DNA endonuclease, thus identifying the catalytic role of the protein in nucleotide excision repair. We suggest that XPG nuclease acts on the single-stranded region created as a result of the combined action of the XPB helicase and XPD helicase at the DNA damage site.
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