Nucleic Acids Research, 1994, Vol. 22, No. 24 5385-5390
© 1994
Articles |
Initiation zone of DNA replication at the aldolase B locus encompasses transcription promoter region
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University Morioka, Iwate 020 1Department of Virology, Iwate Medical School Uchimaru, Morioka, Iwate 020 2Pharmaceutical Research Laboratory, Hitachi Chemicals Co., Ltd Hitachi, Ibaraki 317 3Department of Virology, Nippon University School of Medicine Itabashi, Tokyo 173, Japan
*To whom correspondence should be addressed
Received August 2, 1994. Accepted November 2, 1994.
Aldolase B (AldB) gene Is one of the liver-specific genes, which is activated in the fetal stage. As a first step to Investigate the functional relationship between transcription and DNA replication, we Intended to determine the initiation zone of replication nearest to the AldB gene region. BrdU-labeled nascent DNA was obtained from G1/S arrested hepatoma cells at various times after entering S phase. Hybridization of the newly synthesized, BrdU-labeled DNA with probes corresponding to regions spanning about 26 Kb, revealed that replication zone locates within the AldB gene region. This result, together with the result of hybridization of nascent DNA obtained by alkaline sucrose density-gradient centrifugatIon, suggested that the Initiation zone is located within a more defined region (about 1.0 Kb) containing AldB promoter. In the predicted initiation zone, a purlne-rlch element which shows high homology to known mammalian origin sequences and other replication components are found. Further, autonomously replicating activity of this initiation zone was examined by DNA transfection. The results showed that the predicted Initiation zone confers replication initiation in Cos-1 cells.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Minami, J. Takahashi, A. Suto, Y. Saitoh, and K.-i. Tsutsumi Binding of AlF-C, an Orc1-Binding Transcriptional Regulator, Enhances Replicator Activity of the Rat Aldolase B Origin Mol. Cell. Biol., December 1, 2006; 26(23): 8770 - 8780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hayashida, M. Oda, K. Ohsawa, A. Yamaguchi, T. Hosozawa, R. M. Locksley, M. Giacca, H. Masai, and S. Miyatake Replication Initiation from a Novel Origin Identified in the Th2 Cytokine Cluster Locus Requires a Distant Conserved Noncoding Sequence J. Immunol., May 1, 2006; 176(9): 5446 - 5454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gomez and N. Brockdorff Heterochromatin on the inactive X chromosome delays replication timing without affecting origin usage PNAS, May 4, 2004; 101(18): 6923 - 6928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Price, M. Allarakhia, N. Cossons, T. Nielsen, M. Diaz-Perez, P. Friedlander, L. Tao, and M. Zannis-Hadjopoulos Identification of a cis-Element That Determines Autonomous DNA Replication in Eukaryotic Cells J. Biol. Chem., May 23, 2003; 278(22): 19649 - 19659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Saitoh, S. Miyagi, H. Ariga, and K.-i. Tsutsumi Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene Nucleic Acids Res., December 1, 2002; 30(23): 5205 - 5212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-H. Chen, W.-B. Tseng, Y. Chu, and M.-T. Hsu Interference of the Simian Virus 40 Origin of Replication by the Cytomegalovirus Immediate Early Gene Enhancer: Evidence for Competition of Active Regulatory Chromatin Conformation in a Single Domain Mol. Cell. Biol., June 1, 2000; 20(11): 4062 - 4074. [Abstract] [Full Text]
|
|
|
|
|
|
T. Kobayashi, T. Rein, and M. L. DePamphilis Identification of Primary Initiation Sites for DNA Replication in the Hamster Dihydrofolate Reductase Gene Initiation Zone Mol. Cell. Biol., June 1, 1998; 18(6): 3266 - 3277. [Abstract] [Full Text]
|
|