Nucleic Acids Research, 1994, Vol. 22, No. 3 262-269
© 1994
MOLECULAR BIOLOGY |
Target site choice of the related transposable elements Tc1 and Tc3 of Caenorhabditis elegans
The Netherlands Cancer Institute, Division of Molecular Biology Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
*To whom correspondence should be addressed
Received November 16, 1993. Accepted January 4, 1993.
We have investigated the target choice of the related transposable elements Tc1 and Tc3 of the nematode C.elegans. The exact locations of 204 independent Tc1 insertions and 166 Tc3 insertions in an 1 kbp region of the genome were determined. There was no phenotyplc selection for the insertions. All insertions were into the sequence TA. Both elements have a strong preference for certain positions in the 1 kbp region. Hot sites for integration are not clustered or regularly spaced. The orientation of the integrated transposon has no effect on the distribution pattern. We tested several explanations for the target site preference. If simple structural features of the DNA (e.g. bends) would mark hot sites, we would expect the patterns of the two related transposons Tc1 and Tc3 to be similar; however we found them to be completely different. Furthermore we found that the sequence at the donor site has no effect on the choice of the new insertion site, because the insertion pattern of a transposon that jumps from a transgenlc donor site is identical to the insertion pattern of transposons jumping from endogenous genomic donor sites. The most likely explanation for the target choice is therefore that the primary sequence of the target site is recognized by the transposase. However, alignment of the Tc1 and Tc3 integration sites does not reveal a strong consensus sequence for either transposon.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. S. Linheiro and C. M. Bergman Testing the palindromic target site model for DNA transposon insertion using the Drosophila melanogaster P-element Nucleic Acids Res., October 1, 2008; (2008) gkn563v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Geurts, C. S. Hackett, J. B. Bell, T. L. Bergemann, L. S. Collier, C. M. Carlson, D. A. Largaespada, and P. B. Hackett Structure-based prediction of insertion-site preferences of transposons into chromosomes. Nucleic Acids Res., January 1, 2006; 34(9): 2803 - 2811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rizzon, E. Martin, G. Marais, L. Duret, L. Segalat, and C. Biemont Patterns of Selection Against Transposons Inferred From the Distribution of Tc1, Tc3 and Tc5 Insertions in the mut-7 Line of the Nematode Caenorhabditis elegans Genetics, November 1, 2003; 165(3): 1127 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Carlson, A. J. Dupuy, S. Fritz, K. J. Roberg-Perez, C. F. Fletcher, and D. A. Largaespada Transposon Mutagenesis of the Mouse Germline Genetics, September 1, 2003; 165(1): 243 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Urasaki, Y. Sekine, and E. Ohtsubo Transposition of Cyanobacterium Insertion Element ISY100 in Escherichia coli J. Bacteriol., September 15, 2002; 184(18): 5104 - 5112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. O. Tosi and S. M. Beverley cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics Nucleic Acids Res., February 1, 2000; 28(3): 784 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Migheli, R. Laugé, J.-M. Davière, C. Gerlinger, F. Kaper, T. Langin, and M.-J. Daboussi Transposition of the Autonomous Fot1 Element in the Filamentous Fungus Fusarium oxysporum Genetics, March 1, 1999; 151(3): 1005 - 1013. [Abstract] [Full Text]
|
|
|
|
|
|
Analysis of osm-6, a Gene That Affects Sensory Cilium Structure and Sensory Neuron Function in Caenorhabditis elegans Genetics, January 1, 1998; 148(1): 187 - 200.
|
|
|
|
 |
|
 S. E. Devine, S. L. Chissoe, Y. Eby, R. K. Wilson, and J. D. Boeke A Transposon-Based Strategy for Sequencing Repetitive DNA in Eukaryotic Genomes Genome Res., May 1, 1997; 7(5): 551 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Korswagen, R. M. Durbin, M. T. Smits, and R. H. A. Plasterk Transposon Tc1-derived, sequence-tagged sites in Caenorhabditis elegans as markers for gene mapping PNAS, December 10, 1996; 93(25): 14680 - 14685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J C Vos, I De Baere, and R H Plasterk Transposase is the only nematode protein required for in vitro transposition of Tc1. Genes & Dev., March 15, 1996; 10(6): 755 - 761. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Haapa-Paananen, H. Rita, and H. Savilahti DNA Transposition of Bacteriophage Mu. A QUANTITATIVE ANALYSIS OF TARGET SITE SELECTION IN VITRO J. Biol. Chem., January 18, 2002; 277(4): 2843 - 2851. [Abstract] [Full Text] [PDF]
|
|