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Nucleic Acids Research, 1994, Vol. 22, No. 4 632-638
© 1994

ENZYMOLOGY

DNA ligase I is associated with the 21 S complex of enzymes for DNA synthesis in HeLa cells

Congjun Li+, John Goodchild1 and Earl F. Baril*

Worcester Foundation for Experimental Biology 222 Maple Avenue, Shrewsbury, MA 01545 1Hybridon, Inc One Innovation Drive, Worcester, MA 01605, USA

*To whom correspondence should be addressed

Received October 14, 1993. Accepted January 7, 1994.

Approximately 80% of the DNA ligase activity in HeLa cell extracts is associated with the 21 S enzyme complex that functions in simian virus 40 DNA replication In vitro (Malkas et al., Biochemistry 29, 6362 – 6374., 1990). The DNA ligase associated with the 21 S complex was purified extensively and its physical, enzymlc and immunological properties characterized as DNA ligase I. The association of DNA ligase I with the 21 S complex of enzymes for DNA synthesis provides evidence for the physiological function of this DNA ligase In DNA replication In human cells.

+Present address: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA


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