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Nucleic Acids Research, 1994, Vol. 22, No. 9 1663-1669
© 1994

RNA

Interaction of small ribosomal and transfer RNAs with a protein from Leishmania donovani

Anil Ghosh+, Trina Ghosh, Subhagata Ghosh, Saumitra Das§ and Samit Adhya*

Genetic Engineering Laboratory, Indian Institute of Chemical Biology 4 Raja S.C. Mullick Road, Calcutta 700032, India

*To whom correspondence should be addressed

Received January 11, 1994. Revised March 30, 1994. Accepted March 30, 1994.

Using synthetic antisense RNA from the 5'-untranslated region of the ß-tubulin gene as probe in gel retardation assays, a heat stable RNA-binding factor was identified in promastigotes of the kinetoplastid protozoan Leishmania donovani. The same or similar factors interact with several small ribosomal RNA (srRNA) species and, more weakly, with tRNA, as shown by binding and competition experiments. Deletion analysis indicated involvement of repeated purine-rich motifs on the antisense RNA, in the reaction. Related, conserved motifs occur on at least two of the srRNAs. By a modified Western blot assay, the RNA-binding species was identified as a single, small polypeptide. The activity is apparently specific for the promastigote stage of the parasite, being undetectable in amastigotes. The properties of this RNA-binding factor suggest that it is a novel, previously uncharacterised protein.

+Present addresses: Department of Molecular Virology, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892

§Present addresses: UCLA School of Medicine, 10833 Le Conte Avenue, Los Angeles, CA 90024-1747, USA


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