Skip Navigation

This Article
Right arrow Print PDF (6352K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (33)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by McHugh, P. J.
Right arrow Articles by Knowland, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McHugh, P. J.
Right arrow Articles by Knowland, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 10 1664-1670
© 1995

CHEMISTRY

Novel reagents for chemical cleavage at abasic sites and UV photoproducts in DNA

Peter J. McHugh and John Knowland*

Department of Biochemistry, University of Oxford South Parks Road, Oxford 0X1 3QU, UK

* To whom correspondence should be addressed

Received February 27, 1995. Revised March 31, 1995. Accepted March 31, 1995.

Hot piperidine is often used to cleave abasic and UV-irradiated DNA at the sites of damage. It can inflict non-specific damage on DNA, probably because it Is a strong base and creates significant concentrations of hydroxyl ions which can attack purines and pyrimi-dines. We show that several other amines can cleave abasic DNA at or near neutral pH without non-specific damage. One diamine, N,N-dimethylethylenediamine, efficiently cleaves abasic DNA at pH 7.4 by either ß- or ß,{delta}-elimlnation, depending on temperature. Using end-labelled oligonucieotides we show that cleavage depends mainly on elimination reactions, but that 4',5'-cycllzation is also significant. This reagent also cleaves at photoproducts induced by (JVC and UVB, producing the same overall pattern as piperidine, but with no non-specific damage. It should prove valuable in locating low levels of photoproducts in DNA, such as those induced by natural sunlight.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. K. Saini, K. Maithal, P. Chand, S. Chowdhury, R. Vohra, A. Goyal, G. P. Dubey, P. Chopra, R. Chandra, A. K. Tyagi, et al.
Nuclear Localization and in Situ DNA Damage by Mycobacterium tuberculosis Nucleoside-diphosphate Kinase
J. Biol. Chem., November 26, 2004; 279(48): 50142 - 50149.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Higurashi, T. Ohtsuki, A. Inase, R. Kusumoto, C. Masutani, F. Hanaoka, and S. Iwai
Identification and Characterization of an Intermediate in the Alkali Degradation of (6-4) Photoproduct-containing DNA
J. Biol. Chem., December 19, 2003; 278(51): 51968 - 51973.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. T. Hwang, K. A. Tallman, and M. M. Greenberg
The reactivity of the 2-deoxyribonolactone lesion in single-stranded DNA and its implication in reaction mechanisms of DNA damage and repair
Nucleic Acids Res., October 1, 1999; 27(19): 3805 - 3810.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.