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Nucleic Acids Research, 1995, Vol. 23, No. 19 3894-3900
© 1995

CHEMISTRY

Cleavage of double-stranded DNA by ‘metalloporphyrin-linker oligonucleotide’ molecules: influence of the linker

Pascal Bigey, Geneviève Pratviel and Bernard Meunier*

Laboratoire de Chimie de Coordination du CNRS 205 route de Narbonne, 31077 Toulouse cedex, France

*To whom correspondence should be addressed

Received June 29, 1995. Revised August 24, 1995. Accepted August 24, 1995.

Manganese porphyrin-linker-triple-helix-forming oligonucleotide molecules were prepared and their ability to cleave in vitro a double-stranded DNA target present in the HIV-1 genome was studied. The nature of the linker is a determining factor of the cleavage efficiency. Cleavage yields as high as 80% were observed when the linker was a spermine residue and in the absence of a large excess of free spermlne known to stabilize triplex structures. The hydrophobic nature of aliphatic diamine linker modified the cleaver-DNA interactions and reduced the efficiency of DNA cleavage.


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