Skip Navigation

This Article
Right arrow Print PDF (564K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gut, I. G.
Right arrow Articles by Beck, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gut, I. G.
Right arrow Articles by Beck, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1995, Vol. 23, No. 8 1367-1373
© 1995

METHODS

A procedure for selective DNA alkylation and detection by mass spectrometry

Ivo G. Gut* and Stephan Beck

DNA Sequencing Laboratory, imperial Cancer Research Fund PO Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

*To whom correspondence should be addressed

Received December 22, 1994. Revised March 9, 1995. Accepted March 9, 1995.

A method which improves the detectability of DNA by mass spectrometry is presented. By quantitatively alkylating the backbone of phosphorothioate oligonucleotides the problems of gas phase ion generation by matrix assisted laser desorption ionization can be controlled. We have developed a selective alkylating protocol for phosphorothioate oligonucleotides whichis a facile way of generating non-ionic nucleic acids. A variety of alkylating agents was studied and their kinetics were monitored in a gel electrophoretic assay and by mass spectrometry.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Sauer, D. H. Gelfand, F. Boussicault, K. Bauer, F. Reichert, and I. G. Gut
Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry
Nucleic Acids Res., March 1, 2002; 30(5): e22 - e22.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Sauer, D. Lechner, K. Berlin, C. Plancon, A. Heuermann, H. Lehrach, and I. G. Gut
Full flexibility genotyping of single nucleotide polymorphisms by the GOOD assay
Nucleic Acids Res., December 1, 2000; 28(23): e100 - e100.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Berger, Y. Wu, A. K. Ogawa, D. L. McMinn, P. G. Schultz, and F. E. Romesberg
Universal bases for hybridization, replication and chain termination
Nucleic Acids Res., August 1, 2000; 28(15): 2911 - 2914.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Sauer, D. Lechner, K. Berlin, H. Lehrach, J.-L. Escary, N. Fox, and I. G. Gut
A novel procedure for efficient genotyping of single nucleotide polymorphisms
Nucleic Acids Res., March 1, 2000; 28(5): e13 - e13.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.