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Nucleic Acids Research, 1995, Vol. 23, No. 9 1614-1620
© 1995

MOLECULAR BIOLOGY

8-Methoxypsoralen photoinduced plasmid-chromosome recombination in Saccharomyces cerevisiae using a centromeric vector

Lisiane B. Meira*,, Joao Antonio Pêgas Henriques1 and Nieve Magaña-Schwencke

Institut Curie, Section Biologie URA 1292 CNRS, 26 rue d'Ulm, F-75231 Paris Cedex 05, France 1Laboratório de Radiobiologia Molecular, Centra de Biotecnologia UFRGS, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, RS, Brazil

* To whom correspondence should be addressed at present address: University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235-9072, USA

Received November 21, 1994. Accepted March 10, 1995.

The characterization of a new system to study the induction of plasmid-chromosome recombination is described. Single-stranded and double-stranded centromeric vectors bearing 8-methoxypsoralen photoinduced lesions were used to transform a wild-type yeast strain bearing the leu2-3,112 marker. Using the SSCP methodology and DNA sequencing, it was demonstrated that repair of the lesions in plasmid DNA was mainly due to conversion of the chromosomal allele to the plasmid DNA.


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