Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (129K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dagle, J.
Right arrow Articles by Weeks, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dagle, J.
Right arrow Articles by Weeks, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 11 2143-2149, Copyright © 1996 by Oxford University Press

ARTICLES

Positively charged oligonucleotides overcome potassium-mediated inhibition of triplex DNA formation

JM Dagle and DL Weeks
Department of Pediatrics, The University of Iowa, Iowa City 52242, USA.

The formation of triplex DNA using unmodified, purine-rich oligonucleotides (ODNs) is inhibited by physiologic levels of potassium. Changing negative phosphodiester bonds in a triplex forming oligonucleotide (TFO) to neutral linkages causes a small increase in triplex formation. When phosphodiester bonds in a TFO are converted to positively-charged linkages the formation of triplex DNA increases dramatically. In the absence of KCl, a 17mer TFO containing 11 positively-charged linkages at a concentration of 0.2 microM converts essentially all of a 30 bp target duplex to a triplex. Less than 15% of the target duplex is shifted by 2 microMolar of the unmodified TFO. In 130 mM KCl, triplex formation is undetectable using the unmodified TFO, while triplex formation is nearly complete with 2 microM positively- charged TFO. With increasing potassium, TFOs containing a higher proportion of modified linkages show enhanced triplex formation compared with those less modified. In contrast with unmodified TFOs, triplex formation with more heavily modified TFOs can occur in the absence of divalent cations. We conclude that replacement of phosphodiester bonds with positively-charged phosphoramidate linkages results in more efficient triplex formation, suggesting that these compounds may prove useful for in vivo applications.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Paramasivam, S. Cogoi, V. V. Filichev, N. Bomholt, E. B. Pedersen, and L. E. Xodo
Purine twisted-intercalating nucleic acids: a new class of anti-gene molecules resistant to potassium-induced aggregation
Nucleic Acids Res., June 1, 2008; 36(10): 3494 - 3507.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Murphy, R. Eritja, and G. Redmond
Monitoring denaturation behaviour and comparative stability of DNA triple helices using oligonucleotide-gold nanoparticle conjugates
Nucleic Acids Res., April 23, 2004; 32(7): e65 - e65.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. G. Uil, H. J. Haisma, and M. G. Rots
Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities
Nucleic Acids Res., November 1, 2003; 31(21): 6064 - 6078.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Michel, C. Martinand-Mari, F. Debart, B. Lebleu, I. Robbins, and J.-J. Vasseur
Cationic phosphoramidate {alpha}-oligonucleotides efficiently target single-stranded DNA and RNA and inhibit hepatitis C virus IRES-mediated translation
Nucleic Acids Res., September 15, 2003; 31(18): 5282 - 5290.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Vasquez, J. M. Dagle, D. L. Weeks, and P. M. Glazer
Chromosome Targeting at Short Polypurine Sites by Cationic Triplex-forming Oligonucleotides
J. Biol. Chem., October 12, 2001; 276(42): 38536 - 38541.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. P. Knauert and P. M. Glazer
Triplex forming oligonucleotides: sequence-specific tools for gene targeting
Hum. Mol. Genet., October 1, 2001; 10(20): 2243 - 2251.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. M. Dagle, J. L. Littig, L. B. Sutherland, and D. L. Weeks
Targeted elimination of zygotic messages in Xenopus laevis embryos by modified oligonucleotides possessing terminal cationic linkages
Nucleic Acids Res., May 15, 2000; 28(10): 2153 - 2157.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Bailey and D. L. Weeks
Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes
Nucleic Acids Res., March 1, 2000; 28(5): 1154 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F.-L. M. Lin, A. Majumdar, L. C. Klotz, A. P. Reszka, S. Neidle, and M. M. Seidman
Stability of DNA Triplexes on Shuttle Vector Plasmids in the Replication Pool in Mammalian Cells
J. Biol. Chem., December 8, 2000; 275(50): 39117 - 39124.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.