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Nucleic Acids Research, Vol 24, Issue 11 2190-2191, Copyright © 1996 by Oxford University Press

ARTICLES

Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers

NJ Lench, A Norris, A Bailey, A Booth and AF Markham
Molecular Medicine Unit, Clinical Sciences Building, St James's University Hospital, University of Leeds, UK.

We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR amplification using a vectorette-specific primer in combination with one of a panel of anchored dinucleotide repeat primers. The target DNA to be screened for microsatellite sequences can be from YAC, P1, cosmid, bacteriophage or plasmid clones. We have used this technique to isolate novel, polymorphic microsatellite repeats from clones containing the amelogenin gene (AMGX) located on human chromosome Xp22.3.
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