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© 1996 Oxford University Press 2192-2194

Footnote

Print-capture PCR: a simple and highly sensitive method for the detection of Plum pox virus (PPV) in plant tissues

Print-capture PCR: a simple and highly sensitive method for the detection of Plum pox virus (PPV) in plant tissues Antonio Olmos , Miguel Angel Dasí , Thierry Candresse 1 and Mariano Cambra*

Departamento de Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (I.V.I.A.), Apartado oficial, 46113 Moncada, Valencia , Spain and 1 Station de Pathologie Végétale, INRA, BP81, 33883 Villenave d'Ornon , France

Received February 9, 1996; Revised and Accepted April 15, 1996

Plum pox virus (PPV) is the causal agent of sharka disease of stone fruit trees which is considered to be one of the most serious plant diseases. Sensitive techniques are needed for its diagnosis. Immunosorbent assays based on the use of monoclonal antibodies ( 1 ) and different PCR techniques have been described with or without previous immunocapture ( 2 , 3 ). In all instances plant extracts are needed, even . . . [Full Text of this Article]

ACKNOWLEDGEMENTS

REFERENCES


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