©
1996 Oxford University Press
2460-2462
Footnote
`Long distance sequencer' method; a novel strategy for large DNA sequencing
projects
`Long distance sequencer' method; a novel strategy for large DNA sequencing projects
Koichi
Hagiwara
and
Curtis C.
Harris*
Laboratory of Human Carcinogenesis, National Cancer Institute, 37 Convent Drive,
Bethesda
, MD 20892,
USA
Received January 29, 1996;
Revised and Accepted April 23, 1996
Every DNA sequencing project involves two steps: (i) making suitable templates for all the regions to be sequenced; and (ii) running sequencing reactions and electrophoresis. The latter step can be
automated by use of workstations and autosequencers. The former step requires
careful experimental design and laborious DNA manipulations such as the
construction of nested deletion mutants (
1
). This is often the limiting step in large sequencing projects. The `shot-gun' method eliminates this complicated DNA manipulations (
1
), but many recombinant clones must be sequenced because of the random nature of
this procedure. . . . [Full Text of this Article]
ACKNOWLEDGEMENTS
REFERENCES
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