Nucleic Acids Research, Vol 24, Issue 13 2519-2524, Copyright © 1996 by Oxford University Press
U Guldener, S Heck, T Fielder, J Beinhauer and JH Hegemann
The dominant kanr marker gene plays an important role in gene disruption
experiments in budding yeast, as this marker can be used in a variety of
yeast strains lacking the conventional yeast markers. We have developed a
loxP-kanMX-loxP gene disruption cassette, which combines the advantages of
the heterologous kanr marker with those from the Cre-lox P recombination
system. This disruption cassette integrates with high efficiency via
homologous integration at the correct genomic locus (routinely 70%). Upon
expression of the Cre recombinase the kanMX module is excised by an
efficient recombination between the loxP sites, leaving behind a single
loxP site at the chromosomal locus. This system allows repeated use of the
kanr marker gene and will be of great advantage for the functional analysis
of gene families.
ARTICLES
A new efficient gene disruption cassette for repeated use in budding yeast
Institut fur Mikrobiologie und Molekularbiologie, Justus-Liebig- Universitat Giessen, Giessen, Germany.
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 G. Hasenbrink, A. Sievernich, L. Wildt, J. Ludwig, and H. Lichtenberg-Frate Estrogenic effects of natural and synthetic compounds including tibolone assessed in Saccharomyces cerevisiae expressing the human estrogen {alpha} and {beta} receptors FASEB J, July 1, 2006; 20(9): 1552 - 1554. [Abstract] [Full Text] [PDF]
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U. C. Tran, B. Marbois, P. Gin, M. Gulmezian, T. Jonassen, and C. F. Clarke Complementation of Saccharomyces cerevisiae coq7 Mutants by Mitochondrial Targeting of the Escherichia coli UbiF Polypeptide: TWO FUNCTIONS OF YEAST COQ7 POLYPEPTIDE IN COENZYME Q BIOSYNTHESIS J. Biol. Chem., June 16, 2006; 281(24): 16401 - 16409. [Abstract] [Full Text] [PDF]
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B. Poppenberger, F. Berthiller, H. Bachmann, D. Lucyshyn, C. Peterbauer, R. Mitterbauer, R. Schuhmacher, R. Krska, J. Glossl, and G. Adam Heterologous Expression of Arabidopsis UDP-Glucosyltransferases in Saccharomyces cerevisiae for Production of Zearalenone-4-O-Glucoside. Appl. Envir. Microbiol., June 1, 2006; 72(6): 4404 - 4410. [Abstract] [Full Text] [PDF]
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G. Soveral, A. Veiga, M. C. Loureiro-Dias, A. Tanghe, P. Van Dijck, and T. F. Moura Water channels are important for osmotic adjustments of yeast cells at low temperature. Microbiology, May 1, 2006; 152(Pt 5): 1515 - 1521. [Abstract] [Full Text] [PDF]
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G. Wang and R. J. Deschenes Plasma Membrane Localization of Ras Requires Class C Vps Proteins and Functional Mitochondria in Saccharomyces cerevisiae Mol. Cell. Biol., April 15, 2006; 26(8): 3243 - 3255. [Abstract] [Full Text] [PDF]
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S. Padilla-Lopez and D. A. Pearce Saccharomyces cerevisiae Lacking Btn1p Modulate Vacuolar ATPase Activity to Regulate pH Imbalance in the Vacuole J. Biol. Chem., April 14, 2006; 281(15): 10273 - 10280. [Abstract] [Full Text] [PDF]
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V. Voronkova, N. Kacherovsky, C. Tachibana, D. Yu, and E. T. Young Snf1-Dependent and Snf1-Independent Pathways of Constitutive ADH2 Expression in Saccharomyces cerevisiae Genetics, April 1, 2006; 172(4): 2123 - 2138. [Abstract] [Full Text] [PDF]
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H. Singh, A. M. Erkine, S. B. Kremer, H. M. Duttweiler, D. A. Davis, J. Iqbal, R. R. Gross, and D. S. Gross A Functional Module of Yeast Mediator That Governs the Dynamic Range of Heat-Shock Gene Expression Genetics, April 1, 2006; 172(4): 2169 - 2184. [Abstract] [Full Text] [PDF]
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W. Li, L. Sun, Q. Liang, J. Wang, W. Mo, and B. Zhou Yeast AMID Homologue Ndi1p Displays Respiration-restricted Apoptotic Activity and Is Involved in Chronological Aging Mol. Biol. Cell, April 1, 2006; 17(4): 1802 - 1811. [Abstract] [Full Text] [PDF]
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J. D. Irvin and B. F. Pugh Genome-wide Transcriptional Dependence on TAF1 Functional Domains J. Biol. Chem., March 10, 2006; 281(10): 6404 - 6412. [Abstract] [Full Text] [PDF]
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P. Ternes, P. Sperling, S. Albrecht, S. Franke, J. M. Cregg, D. Warnecke, and E. Heinz Identification of Fungal Sphingolipid C9-methyltransferases by Phylogenetic Profiling J. Biol. Chem., March 3, 2006; 281(9): 5582 - 5592. [Abstract] [Full Text] [PDF]
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A. ZHELKOVSKY, Y. TACAHASHI, T. NASSER, X. HE, U. STERZER, T. H. JENSEN, H. DOMDEY, and C. MOORE The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae RNA, March 1, 2006; 12(3): 435 - 445. [Abstract] [Full Text] [PDF]
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F. J. van Werven and H. Th. M. Timmers The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation Nucleic Acids Res., February 25, 2006; 34(4): e33 - e33. [Abstract] [Full Text] [PDF]
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S. M. G. Saerens, K. J. Verstrepen, S. D. M. Van Laere, A. R. D. Voet, P. Van Dijck, F. R. Delvaux, and J. M. Thevelein The Saccharomyces cerevisiae EHT1 and EEB1 Genes Encode Novel Enzymes with Medium-chain Fatty Acid Ethyl Ester Synthesis and Hydrolysis Capacity J. Biol. Chem., February 17, 2006; 281(7): 4446 - 4456. [Abstract] [Full Text] [PDF]
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Z. Hu, B. Potthoff, C. P. Hollenberg, and M. Ramezani-Rad Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae J. Cell Sci., January 15, 2006; 119(2): 326 - 338. [Abstract] [Full Text] [PDF]
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Y.-S. Jin, H. Alper, Y.-T. Yang, and G. Stephanopoulos Improvement of Xylose Uptake and Ethanol Production in Recombinant Saccharomyces cerevisiae through an Inverse Metabolic Engineering Approach Appl. Envir. Microbiol., December 1, 2005; 71(12): 8249 - 8256. [Abstract] [Full Text] [PDF]
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S. Lebaron, C. Froment, M. Fromont-Racine, J.-C. Rain, B. Monsarrat, M. Caizergues-Ferrer, and Y. Henry The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles Mol. Cell. Biol., November 1, 2005; 25(21): 9269 - 9282. [Abstract] [Full Text] [PDF]
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J. Zhao, J. Herrera-Diaz, and D. S. Gross Domain-Wide Displacement of Histones by Activated Heat Shock Factor Occurs Independently of Swi/Snf and Is Not Correlated with RNA Polymerase II Density Mol. Cell. Biol., October 15, 2005; 25(20): 8985 - 8999. [Abstract] [Full Text] [PDF]
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K. Melcher Mutational Hypersensitivity of a Gene Regulatory Protein: Saccharomyces cerevisiae Gal80p Genetics, October 1, 2005; 171(2): 469 - 476. [Abstract] [Full Text] [PDF]
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