Department of Pathology, Yale University School of Medicine, PO Box 208023, Lauder Hall 219C, New Haven , CT 06520-8023, USA

Received January 2, 1996; Revised and Accepted May 2, 1996

PCR has become a powerful tool for genetic analysis and many applications for gene sequence quantitation are based on this technology ( 1 - 3 ). Standardized reaction conditions require accurate quantitation of input DNA as well as optimization of chemical and cycling parameters. In this study we discuss PicoGreen^tm (Molecular Probes, Eugene, OR) fluorescence enhancement as a useful assay for template DNA quantitation and PCR product formation.

Spectrophotometry is the principal method for evaluating quantity and quality of nucleic acids. In aqueous solution, DNA has maximal absorbance near 260 nm with . . . [Full Text of this Article]

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