Nucleic Acids Research, Vol 24, Issue 14 2760-2766, Copyright © 1996 by Oxford University Press
A Lubys, J Lubiene, S Kulakauskas, K Stankevicius, A Timinskas and A Janulaitis
The genomic region encoding the type IIS restriction-modification (R-M)
system HphI (enzymes recognizing the asymmetric sequence 5'-GGTGA-3'/5'-
TCACC-3') from Haemophilus parahaemolyticus were cloned into Escherichia
coli and sequenced. Sequence analysis of the R-M HphI system revealed three
adjacent genes aligned in the same orientation: a cytosine 5
methyltransferase (gene hphIMC), an adenine N6 methyltransferase (hphIMA)
and the HphI restriction endonuclease (gene hphIR). Either
methyltransferase is capable of protecting plasmid DNA in vivo against the
action of the cognate restriction endonuclease. hphIMA methylation renders
plasmid DNA resistant to R.Hindill at overlapping sites, suggesting that
the adenine methyltransferase modifies the 3'-terminal A residue on the
GGTGA strand. Strong homology was found between the N-terminal part of the
m6A methyltransferasease and an unidentified reading frame interrupted by
an incomplete gaIE gene of Neisseria meningitidis. The HphI R-M genes are
flanked by a copy of a 56 bp direct nucleotide repeat on each side. Similar
sequences have also been identified in the non-coding regions of
H.influenzae Rd DNA. Possible involvement of the repeat sequences in the
mobility of the HphI R-M system is discussed.
ARTICLES
Cloning and analysis of the genes encoding the type IIS restriction- modification system HphI from Haemophilus parahaemolyticus
Institute of Biotechnology, Vilnius, Lithuania.
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