Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (112K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (73)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lafontaine, D
Right arrow Articles by Tollervey, D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lafontaine, D
Right arrow Articles by Tollervey, D
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 17 3469-3471, Copyright © 1996 by Oxford University Press

ARTICLES

One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins

D Lafontaine and D Tollervey
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
J. Dukanovic, K. S. Dimmer, N. Bonnefoy, K. Krumpe, and D. Rapaport
Genetic and Functional Interactions between the Mitochondrial Outer Membrane Proteins Tom6 and Sam37
Mol. Cell. Biol., November 15, 2009; 29(22): 5975 - 5988.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
C. Dez, M. Dlakic, and D. Tollervey
Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation
RNA, September 1, 2007; 13(9): 1516 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
I. V. Rosado, C. Dez, S. Lebaron, M. Caizergues-Ferrer, Y. Henry, and J. de la Cruz
Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) Reveals a Low-Molecular-Mass Complex Containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p Involved in Early Steps of 60S Ribosomal Subunit Biogenesis
Mol. Cell. Biol., February 15, 2007; 27(4): 1207 - 1221.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
R. Bax, H. R. Vos, H. A. Raue, and J. C. Vos
Saccharomyces cerevisiae Sof1p Associates with 35S Pre-rRNA Independent from U3 snoRNA and Rrp5p
Eukaryot. Cell, March 1, 2006; 5(3): 427 - 434.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Milligan, C. Torchet, C. Allmang, T. Shipman, and D. Tollervey
A Nuclear Surveillance Pathway for mRNAs with Defective Polyadenylation
Mol. Cell. Biol., November 15, 2005; 25(22): 9996 - 10004.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Kufel, C. Bousquet-Antonelli, J. D. Beggs, and D. Tollervey
Nuclear Pre-mRNA Decapping and 5' Degradation in Yeast Require the Lsm2-8p Complex
Mol. Cell. Biol., November 1, 2004; 24(21): 9646 - 9657.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Colau, M. Thiry, V. Leduc, R. Bordonne, and D. L. J. Lafontaine
The Small Nucle(ol)ar RNA Cap Trimethyltransferase Is Required for Ribosome Synthesis and Intact Nucleolar Morphology
Mol. Cell. Biol., September 15, 2004; 24(18): 7976 - 7986.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Dez, C. Froment, J. Noaillac-Depeyre, B. Monsarrat, M. Caizergues-Ferrer, and Y. Henry
Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification
Mol. Cell. Biol., July 15, 2004; 24(14): 6324 - 6337.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Herlan, C. Bornhovd, K. Hell, W. Neupert, and A. S. Reichert
Alternative topogenesis of Mgm1 and mitochondrial morphology depend on ATP and a functional import motor
J. Cell Biol., April 26, 2004; 165(2): 167 - 173.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Kufel, C. Allmang, L. Verdone, J. Beggs, and D. Tollervey
A complex pathway for 3' processing of the yeast U3 snoRNA
Nucleic Acids Res., December 1, 2003; 31(23): 6788 - 6797.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Mitchell, E. Petfalski, R. Houalla, A. Podtelejnikov, M. Mann, and D. Tollervey
Rrp47p Is an Exosome-Associated Protein Required for the 3' Processing of Stable RNAs
Mol. Cell. Biol., October 1, 2003; 23(19): 6982 - 6992.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Sandmann, J. M. Herrmann, J. Dengjel, H. Schwarz, and A. Spang
Suppression of Coatomer Mutants by a New Protein Family with COPI and COPII Binding Motifs in Saccharomyces cerevisiae
Mol. Biol. Cell, August 1, 2003; 14(8): 3097 - 3113.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. H. Geerlings, A. W. Faber, M. D. Bister, J. C. Vos, and H. A. Raue
Rio2p, an Evolutionarily Conserved, Low Abundant Protein Kinase Essential for Processing of 20 S Pre-rRNA in Saccharomyces cerevisiae
J. Biol. Chem., June 13, 2003; 278(25): 22537 - 22545.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Fritz, N. Weinbach, and B. Westermann
Mdm30 Is an F-Box Protein Required for Maintenance of Fusion-competent Mitochondria in Yeast
Mol. Biol. Cell, June 1, 2003; 14(6): 2303 - 2313.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Fatica, M. Oeffinger, M. Dlakic, and D. Tollervey
Nob1p Is Required for Cleavage of the 3' End of 18S rRNA
Mol. Cell. Biol., March 1, 2003; 23(5): 1798 - 1807.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
M. ALBERS, A. DIMENT, M. MURARU, C. S. RUSSELL, and J. D. BEGGS
Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors
RNA, January 1, 2003; 9(1): 138 - 150.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Dez, J. Noaillac-Depeyre, M. Caizergues-Ferrer, and Y. Henry
Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs
Mol. Cell. Biol., October 15, 2002; 22(20): 7053 - 7065.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Kufel, C. Allmang, L. Verdone, J. D. Beggs, and D. Tollervey
Lsm Proteins Are Required for Normal Processing of Pre-tRNAs and Their Efficient Association with La-Homologous Protein Lhp1p
Mol. Cell. Biol., July 15, 2002; 22(14): 5248 - 5256.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Gray and S. M. Honigberg
Effect of chromosomal locus, GC content and length of homology on PCR-mediated targeted gene replacement in Saccharomyces
Nucleic Acids Res., December 15, 2001; 29(24): 5156 - 5162.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Maurer, M. Redd, J. Solsbacher, F. R. Bischoff, M. Greiner, A. V. Podtelejnikov, M. Mann, K. Stade, K. Weis, and G. Schlenstedt
The Nuclear Export Receptor Xpo1p Forms Distinct Complexes with NES Transport Substrates and the Yeast Ran Binding Protein 1 (Yrb1p)
Mol. Biol. Cell, March 1, 2001; 12(3): 539 - 549.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
R. Bordonné
Functional Characterization of Nuclear Localization Signals in Yeast Sm Proteins
Mol. Cell. Biol., November 1, 2000; 20(21): 7943 - 7954.
[Abstract] [Full Text]

Home page
 Genome ResHome page
J. L. Hartley, G. F. Temple, and M. A. Brasch
DNA Cloning Using In Vitro Site-Specific Recombination
Genome Res., November 1, 2000; 10(11): 1788 - 1795.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
A. Colley, J. D. Beggs, D. Tollervey, and D. L. J. Lafontaine
Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3
Mol. Cell. Biol., October 1, 2000; 20(19): 7238 - 7246.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
J. Kufel, C. Allmang, G. Chanfreau, E. Petfalski, D. L. J. Lafontaine, and D. Tollervey
Precursors to the U3 Small Nucleolar RNA Lack Small Nucleolar RNP Proteins but Are Stabilized by La Binding
Mol. Cell. Biol., August 1, 2000; 20(15): 5415 - 5424.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
D. L. J. Lafontaine and D. Tollervey
Synthesis and Assembly of the Box C+D Small Nucleolar RNPs
Mol. Cell. Biol., April 15, 2000; 20(8): 2650 - 2659.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
C. Allmang, P. Mitchell, E. Petfalski, and D. Tollervey
Degradation of ribosomal RNA precursors by the exosome
Nucleic Acids Res., April 15, 2000; 28(8): 1684 - 1691.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Allmang, E. Petfalski, A. Podtelejnikov, M. Mann, D. Tollervey, and P. Mitchell
The yeast exosome and human PM-Scl are related complexes of 3' right-arrow 5' exonucleases
Genes & Dev., August 15, 1999; 13(16): 2148 - 2158.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
A. Kaffman, N. M. Rank, and E. K. O'Shea
Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121
Genes & Dev., September 1, 1998; 12(17): 2673 - 2683.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
D. L. J. Lafontaine, T. Preiss, and D. Tollervey
Yeast 18S rRNA Dimethylase Dim1p: a Quality Control Mechanism in Ribosome Synthesis?
Mol. Cell. Biol., April 1, 1998; 18(4): 2360 - 2370.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
D. L.J. Lafontaine, C. Bousquet-Antonelli, Y. Henry, M. Caizergues-Ferrer, and D. Tollervey
The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase
Genes & Dev., February 15, 1998; 12(4): 527 - 537.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
I. Flury, A. Benachour, and A. Conzelmann
YLL031c Belongs to a Novel Family of Membrane Proteins Involved in the Transfer of Ethanolaminephosphate onto the Core Structure of Glycosylphosphatidylinositol Anchors in Yeast
J. Biol. Chem., August 4, 2000; 275(32): 24458 - 24465.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Brouwer, C. Allmang, R. Raijmakers, Y. van Aarssen, W. V. Egberts, E. Petfalski, W. J. van Venrooij, D. Tollervey, and G. J. M. Pruijn
Three Novel Components of the Human Exosome
J. Biol. Chem., February 23, 2001; 276(9): 6177 - 6184.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Yu, H. M. Ellis, E-C. Lee, N. A. Jenkins, N. G. Copeland, and D. L. Court
An efficient recombination system for chromosome engineering in Escherichia coli
PNAS, May 23, 2000; 97(11): 5978 - 5983.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.