Nucleic Acids Research, Vol 24, Issue 20 4078-4083, Copyright © 1996 by Oxford University Press
MN Rao, E Chernokalskaya and DR Schoenberg
Cytoplasmic regulation of the length of poly(A) on mRNA is a well-
characterized process involved in translational control during development.
In contrast, there is no direct in vivo evidence for regulation of the
length of poly(A) added during nuclear pre-mRNA processing in somatic
cells. We previously reported that Xenopus serum albumin [Schoenberg et al.
(1989) Mol. Endocrinol. 3, 805-815] and transferrin [Pastori et al. (1992)
J. Steroid Biochem. Mol. Biol. 42, 649-657], mRNA have exceptionally short
poly(A) tails ranging from 12 to 17 residues, whereas vitellogenin mRNA has
long poly(A). An RT-PCR protocol was adapted to determine the length of
poly(A) added onto pre- mRNA, defined here as that species bearing the
terminal intron. Using this assay we show that vitellogenin pre-mRNA has
the same long poly(A) tail as mature vitellogenin mRNA. In contrast,
albumin pre-mRNA has the same short poly(A) as found on fully-processed
albumin mRNA. These results indicate that the short poly(A) tail on albumin
mRNA results from regulation of poly(A) addition during nuclear 3'
processing.
ARTICLES
Regulated nuclear polyadenylation of Xenopus albumin pre-mRNA
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA.
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