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Nucleic Acids Research, Vol 24, Issue 20 4094-4095, Copyright © 1996 by Oxford University Press

ARTICLES

Genomic analysis of human multigene families using chromosome-specific vectorette PCR

TP Moynihan, AF Markham and PA Robinson
Molecular Medicine Unit, University of Leeds, St James's University Hospital, UK.

We report a technique for the rapid determination of genomic structure of individual members of human interspersed multigene families which circumvents the requirement for genomic clone isolation. In this approach, vectorette libraries were constructed from human/rodent somatic cell hybrid DNA harbouring single members of the gene family. Using these libraries as PCR templates with nested gene-specific primers in combination with a common vectorette primer resulted in the amplification of gene-specific products suitable for the subsequent determination of intron/exon structure. We have applied this technique to characterise members of two gene families.
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