Nucleic Acids Research, Vol 24, Issue 22 4543-4551, Copyright © 1996 by Oxford University Press
DB Jansma, J Archambault, O Mostachfi and JD Friesen
We have determined the location of cis-acting elements that are important
for the expression of RPO21 and RPO22, genes that encode the two largest
subunits of RNA polymerase II (RNAPII) in Saccharomyces cerevisiae. A
series of 5'-end deletions and nucleotide substitutions in the upstream
regions of RPO21 and RPO22 were tested for their effect on the expression
of lacZ fusions of these genes. Deletion of sequences from -723 to -693 in
RPO21, which disrupted two Reb1p-binding sites and an Abf1p-binding site,
resulted in a 10-fold decrease in expression. A T-rich region downstream of
these sites was also important for expression. Deletion of sequences from
-437 to -392 in the RPO22- upstream, which resulted in a 30-fold decrease
in expression, indicated that the Reb1p- and Abf1p-binding sites in this
region were important for RPO22 expression, as was a T-rich sequence
immediately downstream of these sites. The RPO21 and RPO22 upstream regions
were capable of interacting in vitro (gel-mobility-shift assays) with Reb1p
and Abf1p. The similarities in the type and organization of elements in the
upstream regions of RPO21 and RPO22 suggest that expression of these genes
may be regulated coordinately.
ARTICLES
Similar upstream regulatory elements of genes that encode the two largest subunits of RNA polymerase II in Saccharomyces cerevisiae
Department of Genetics, The Hospital for Sick Children, Toronto, Ontario, Canada.
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