Nucleic Acids Research, Vol 24, Issue 22 4594-4596, Copyright © 1996 by Oxford University Press
T Storck, U Kruth, R Kolhekar, R Sprengel and PH Seeburg
Targeting vectors for embryonic stem (ES) cells typically contain a mouse
gene segment of >7 kb with the neo gene inserted for positive selection
of the targeting event. More complex targeting vectors carry additional
genetic elements (e.g. lacZ, loxP, point mutations). Here we use homologous
recombination in yeast to construct targeting vectors for the incorporation
of genetic elements (GEs) into mouse genes. The precise insertion of GEs
into any position of a mouse gene segment cloned in an Escherichia
coli/yeast shuttle vector is directed by short recombinogenic arms (RAs)
flanking the GEs. In this way, complex targeting vectors can be engineered
with considerable ease and speed, obviating extensive gene mapping in
search for suitable restriction sites.
ARTICLES
Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse
Center for Molecular Biology (ZMBH), University of Heidelberg, Germany. storck@sun0.urz.uni-heidelberg.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. R. Thakker, F. Natt, D. Husken, R. Maier, M. Muller, H. van der Putten, D. Hoyer, and J. F. Cryan Neurochemical and behavioral consequences of widespread gene knockdown in the adult mouse brain by using nonviral RNA interference PNAS, December 7, 2004; 101(49): 17270 - 17275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Houghtaling, C. Timmers, M. Noll, M. J. Finegold, S. N. Jones, M. S. Meyn, and M. Grompe Epithelial cancer in Fanconi anemia complementation group D2 (Fancd2) knockout mice Genes & Dev., August 15, 2003; 17(16): 2021 - 2035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ciani, A. Patel, N. D. Allen, and C. ffrench-Constant Mice Lacking the Giant Protocadherin mFAT1 Exhibit Renal Slit Junction Abnormalities and a Partially Penetrant Cyclopia and Anophthalmia Phenotype Mol. Cell. Biol., May 15, 2003; 23(10): 3575 - 3582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Soderling, L. K. Langeberg, J. A. Soderling, S. M. Davee, R. Simerly, J. Raber, and J. D. Scott Loss of WAVE-1 causes sensorimotor retardation and reduced learning and memory in mice PNAS, February 18, 2003; 100(4): 1723 - 1728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. van der Weyden, D. J. Adams, and A. Bradley Tools for targeted manipulation of the mouse genome Physiol Genomics, December 3, 2002; 11(3): 133 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Fuchs, H. Doheny, H. Faulkner, A. Caputi, R. D. Traub, A. Bibbig, N. Kopell, M. A. Whittington, and H. Monyer Genetically altered AMPA-type glutamate receptor kinetics in interneurons disrupt long-range synchrony of gamma oscillation PNAS, March 13, 2001; 98(6): 3571 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hartley, G. F. Temple, and M. A. Brasch DNA Cloning Using In Vitro Site-Specific Recombination Genome Res., November 1, 2000; 10(11): 1788 - 1795. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. R. PICCIOTTO and K. WICKMAN Using Knockout and Transgenic Mice to Study Neurophysiology and Behavior Physiol Rev, October 1, 1998; 78(4): 1131 - 1163. [Abstract] [Full Text] [PDF]
|
|