Nucleic Acids Research, Vol 24, Issue 4 766-774, Copyright © 1996 by Oxford University Press
QL Deng, S Ishii and A Sarai
The c-Myb oncoprotein is known to bind to multiple sites in the promoters
of target genes. We have developed a protocol to screen the binding site of
c-Myb by using the systematic binding data derived form measurements of
binding affinity for oligonucleotide containing a known Myb-binding site
and its complete single mutants. We first applied the method to predict the
binding affinity for the known binding sites and compared with available
experimental data. The predicated binding sites agree with many putative
binding sites of known target promoters. However, there are some binding
sites not predicated by the analysis. These sequences deviate from the
consensus sequence derived from the binding analyses. In the light of the
structure of Myb-DNA complex, these results indicate that different
DNA-binding modes may be used by c-Myb to recognize different classes of
binding sites. We also screened the sequence database for potential
Myb-binding sites, and found sequences of several promoters that have not
been identified experimentally but could be the target for c-Myb.
ARTICLES
Binding site analysis of c-Myb: screening of potential binding sites by using the mutation matrix derived from systematic binding affinity measurements
Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Tsukuba, Ibaraki 305, Japan.
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