Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (235K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Boggon, T.
Right arrow Articles by Leonard, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boggon, T.
Right arrow Articles by Leonard, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 24, Issue 5 951-961, Copyright © 1996 by Oxford University Press

ARTICLES

The crystal structure analysis of d(CGCGAASSCGCG)2, a synthetic DNA dodecamer duplex containing four 4'-thio-2'-deoxythymidine nucleotides

TJ Boggon, EL Hancox, KE McAuley-Hecht, BA Connolly, WN Hunter, T Brown, RT Walker and GA Leonard
Department of Chemistry, University of Manchester, UK.

The crystal structure refinement of the synthetic dodecamer d(CGCGAASSCGCG), where S = 4'-thio-2'-deoxythymidine, has converged at R=0.201 for 2605 reflections with F > 2sigma(F) in the resolution range 8.0-2.4 A for a model consisting of the dodecamer duplex and 66 water molecules. A comparison of its structure with that of the native dodecamer d(CGCGAATTCGCG) has revealed that the major differences between the two structures is a change in the conformation of the sugar- phosphate backbone in the regions at and adjacent to the positions of the modified nucleosides. Examination of the fine structural parameters for each of the structures reveals that the thiosugars adopt a C3'-exo conformation in d(CGCGAASSCGCG), rather than the approximate C1'-exo conformation found for the analogous sugars in the structure of d(CGCGAATTCGCG). The observed differences in structure between the two duplexes may help to explain the enhanced resistance to nuclease digestion of synthetic oligonucleotides containing 4'-thio-2'- deoxynucleotides.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Matsugami, T. Ohyama, M. Inada, N. Inoue, N. Minakawa, A. Matsuda, and M. Katahira
Unexpected A-form formation of 4'-thioDNA in solution, revealed by NMR, and the implications as to the mechanism of nuclease resistance
Nucleic Acids Res., April 1, 2008; 36(6): 1805 - 1812.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Robeyns, P. Herdewijn, and L. Van Meervelt
Influence of the incorporation of a cyclohexenyl nucleic acid (CeNA) residue onto the sequence d(CGCGAATTCGCG)
Nucleic Acids Res., March 1, 2008; 36(5): 1407 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Inoue, N. Minakawa, and A. Matsuda
Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA
Nucleic Acids Res., July 19, 2006; 34(12): 3476 - 3483.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Haeberli, I. Berger, P. S. Pallan, and M. Egli
Syntheses of 4'-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4'-thiocytosine
Nucleic Acids Res., July 18, 2005; 33(13): 3965 - 3975.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.