©
1996 Oxford University Press
981-983
Footnote
High yield photocrosslinking of a 5-iodocytidine (IC) substituted RNA to its associated protein
High yield photocrosslinking of a 5-iodocytidine (IC) substituted RNA to its associated protein
Kristen M.
Meisenheimer
,
Poncho L.
Meisenheimer
,
Michael C.
Willis
and
Tad H.
Koch*
Department of Chemistry and Biochemistry, University of Colorado,
Boulder
, CO 80309-0215,
USA
Received November 24, 1995;
Revised and Accepted January 16, 1996
5-Iodouracil is an excellent chromophore for introduction into both RNA and
DNA for the purpose of achieving high yield photocrosslinking of nucleoprotein complexes (
1
,
2
). Crosslinking yields are often three to five times higher than those achieved
with 5-bromouracil, in part, because the 5-iodouracil chromophore absorbs at longer wavelength and consequently
can be excited more selectively. The highest yields are achieved by irradiating
with a helium cadmium laser emitting at 325 nm (
1
); however, useful yields can also be obtained with a 312 nm transilluminator (
1
. . . [Full Text of this Article]
ACKNOWLEDGEMENTS
REFERENCES
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